Department of Cardiovascular Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Department of Cardiovascular Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Int J Cardiol. 2024 Dec 15;417:132579. doi: 10.1016/j.ijcard.2024.132579. Epub 2024 Sep 19.
To determine cell-free mitochondrial DNA (mt-cfDNA) levels in tachycardia-induced cardiomyopathy (TIC) and non-TIC among atrial fibrillation (AF) cases.
TIC is a reversible cardiomyopathy resulting from tachyarrhythmias, such as AF. The exact cause of TIC is not fully understood, but mitochondrial dysfunction has been reported in a variety of cardiomyopathies and may be involved in TIC as well. AF is recognized to be associated with systemic inflammation, and studies have shown that in patients with AF have elevated levels of mt-cfDNA increased, and this increase is linked to systemic inflammation.
We enrolled 67 patients with TIC (TIC group) and 671 patients without TIC (non-TIC group), who underwent catheter ablation for AF at our hospital between November 2009 and September 2016 and did not meet the exclusion criteria. We performed quantitative PCR analysis of plasma mt-cfDNA and nuclear-cfDNA and compared clinical factors and these measurements between the two groups.
Levels of mt-cfDNA were significantly lower in the TIC group than in the non-TIC group (1110.01 vs. 1918.71 copies/μg plasma, P = 0.027), while levels of nuclear-cfDNA were comparable between these two groups. In particular, mt-cfDNA (P = 0.0003, odds ratio [OR] 2.54), non-paroxysmal AF (P < 0.0001, OR 3.07), and diabetes mellitus (P = 0.006, OR 2.36) were identified as independent factors associated with TIC.
There are lower mt-cfDNA in TIC, and decreased plasma levels of circulating mt-cfDNA may be a new biomarker and involve in related mechanisms for AF associated TIC.
Tachycardia-induced cardiomyopathy (TIC) is a reversible cardiomyopathy caused by tachyarrhythmias, such as atrial fibrillation (AF) tachycardia. The pathogenesis of TIC remains incompletely understood, and there is currently no method to predict its development in patients. In this study, we show that cell-free mitochondrial DNA (mt-cfDNA) levels were significantly lower in the TIC group than in the non-TIC group. Persistent AF, coexisting diabetes mellitus, and decreased mt-cfDNA levels were independently associated with TIC. Decreased mt-cfDNA levels may serve as a novel biomarker for predicting TIC in patients with AF.
在心房颤动（AF）病例中，确定心动过速性心肌病（TIC）和非 TIC 中的无细胞线粒体 DNA（mt-cfDNA）水平。
TIC 是一种由心动过速等心律失常引起的可逆性心肌病。TIC 的确切病因尚不完全清楚，但已有研究报道多种心肌病中线粒体功能障碍的存在，这可能与 TIC 也有关。AF 与全身炎症有关，研究表明，AF 患者的 mt-cfDNA 水平升高，这种增加与全身炎症有关。
我们纳入了 2009 年 11 月至 2016 年 9 月在我院接受导管消融治疗 AF 的 67 例 TIC 患者（TIC 组）和 671 例非 TIC 患者（非 TIC 组），并排除了不符合纳入标准的患者。我们对血浆 mt-cfDNA 和核 cfDNA 进行了定量 PCR 分析，并比较了两组间的临床因素和这些指标。
TIC 组的 mt-cfDNA 水平明显低于非 TIC 组（1110.01 对 1918.71 拷贝/μg 血浆，P=0.027），而两组间核 cfDNA 水平相当。特别是 mt-cfDNA（P=0.0003，比值比[OR]2.54）、非阵发性 AF（P<0.0001，OR3.07）和糖尿病（P=0.006，OR2.36）被确定为与 TIC 相关的独立因素。
TIC 患者的 mt-cfDNA 水平较低，循环血浆中 mt-cfDNA 水平降低可能是 AF 相关 TIC 的一种新的生物标志物，并涉及相关机制。
