Koeda Sota, Fortes Isabel M, Rodríguez-López Maria J, Fernández-Muñoz Rafael, Moriones Enrique
Faculty of Agriculture, Kindai University, Nara 631-8505, Japan.
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM), Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Estación Experimental "La Mayora" E-29750, Algarrobo-Costa, Málaga, Spain.
Plant Dis. 2025 Feb;109(2):399-409. doi: 10.1094/PDIS-06-24-1281-RE. Epub 2025 Feb 5.
Tomato yellow leaf curl virus (TYLCV) is a begomovirus (genus , family ) transmitted persistently by the whitefly . It causes tomato yellow leaf curl disease (TYLCD), resulting in significant yield losses worldwide. TYLCD is controlled mainly by using F hybrid tomato cultivars harboring the TYLCV resistance gene . However, infected -bearing tomato plants accumulate viral DNA, which may eventually lead to the emergence of a resistance-breaking TYLCV variant. Recently, a -resistant tomato line derived from the introgression of type IV leaf glandular trichomes and acylsucrose secretion from wild tomato () was shown to effectively control the spread of TYLCV. In this study, we combined resistance and -based TYLCV resistance to increase the robustness and durability of the TYLCD resistance mediated by in tomato plants. Specifically, we characterized and used a Group 2-like isolate of the Israel strain of TYLCV (TYLCV-IL-G2) that contributes to TYLCD epidemics in southeastern Spain. A comparison with isolates of the previously identified TYLCV variant revealed TYLCV-IL-G2 has a similar host range, but it induces a slightly more severe TYLCD in -bearing tomato plants. Moreover, we demonstrated that acylsucrose-producing -resistant tomato plants can limit the spread of TYLCV-IL-G2 better than a near-isogenic line lacking type IV trichomes and unable to secrete acylsucrose. Pyramiding -based TYLCV resistance and resistance provided by type IV glandular trichomes helped to decrease the effects of TYLCV on -bearing tomato plants as well as the likelihood of TYLCV evolution in infected plants.
番茄黄化曲叶病毒（TYLCV）是一种双生病毒（属，科），由烟粉虱持久传播。它会引发番茄黄化曲叶病（TYLCD），在全球范围内导致显著的产量损失。TYLCD主要通过使用携带TYLCV抗性基因的F杂交番茄品种来控制。然而，携带病毒的番茄植株会积累病毒DNA，这最终可能导致出现打破抗性的TYLCV变体。最近，一种源自野生番茄（）IV型叶腺毛和酰基蔗糖分泌基因渗入的抗TYLCV番茄品系被证明能有效控制TYLCV的传播。在本研究中，我们将基于的抗性和基于的TYLCV抗性相结合，以增强番茄植株中由介导的TYLCD抗性的稳健性和持久性。具体而言，我们鉴定并使用了TYLCV以色列株系的一个类似第2组的分离株（TYLCV-IL-G2），该分离株在西班牙东南部导致TYLCD流行。与先前鉴定的TYLCV变体的分离株进行比较发现，TYLCV-IL-G2具有相似的寄主范围，但它在携带的番茄植株中引发的TYLCD症状略为严重。此外，我们证明，能产生酰基蔗糖的抗番茄植株比缺乏IV型毛状体且不能分泌酰基蔗糖的近等基因系能更好地限制TYLCV-IL-G2的传播。将基于的TYLCV抗性与IV型腺毛提供的抗性相结合，有助于降低TYLCV对携带的番茄植株的影响以及感染植株中TYLCV进化的可能性。
