Resistance to the Insect Vector Enhances the Robustness and Durability of Tomato Yellow Leaf Curl Virus Resistance Conferred by .

Tomato yellow leaf curl virus (TYLCV) is a begomovirus (genus , family ) transmitted persistently by the whitefly . It causes tomato yellow leaf curl disease (TYLCD), resulting in significant yield losses worldwide. TYLCD is controlled mainly by using F hybrid tomato cultivars harboring the TYLCV resistance gene . However, infected -bearing tomato plants accumulate viral DNA, which may eventually lead to the emergence of a resistance-breaking TYLCV variant. Recently, a -resistant tomato line derived from the introgression of type IV leaf glandular trichomes and acylsucrose secretion from wild tomato () was shown to effectively control the spread of TYLCV. In this study, we combined resistance and -based TYLCV resistance to increase the robustness and durability of the TYLCD resistance mediated by in tomato plants. Specifically, we characterized and used a Group 2-like isolate of the Israel strain of TYLCV (TYLCV-IL-G2) that contributes to TYLCD epidemics in southeastern Spain. A comparison with isolates of the previously identified TYLCV variant revealed TYLCV-IL-G2 has a similar host range, but it induces a slightly more severe TYLCD in -bearing tomato plants. Moreover, we demonstrated that acylsucrose-producing -resistant tomato plants can limit the spread of TYLCV-IL-G2 better than a near-isogenic line lacking type IV trichomes and unable to secrete acylsucrose. Pyramiding -based TYLCV resistance and resistance provided by type IV glandular trichomes helped to decrease the effects of TYLCV on -bearing tomato plants as well as the likelihood of TYLCV evolution in infected plants.