Zhang Peipei, Liu Zhilong, Ma Guiqiao, Wang Junwei, Shao Jing, Ma Chaojing, Wang Liping, Ma Chanjuan
Department of Nephrology, The Fifth Clinical Medical College of Shanxi Medical University, Fifth Hospital of Shanxi Medical University, Taiyuan, PR China.
Shanxi Provincial Key Laboratory of Kidney Disease, Shanxi Provincial People's Hospital, Taiyuan, PR China.
Arch Physiol Biochem. 2025 Apr;131(2):285-292. doi: 10.1080/13813455.2024.2407552. Epub 2024 Sep 27.
Diabetic kidney disease (DKD) is one of the common microvascular complications of diabetes, and there are still lack of effective treatments. Huaiqihuang (HQH) is a kind of traditional Chinese medicine mixed preparation, which is mainly made of Trametes robiniophila Murr, Fructus Lycii, and Polygonatum sibiricumhas. It has been shown to be effective in the treatment of DKD, but the specific mechanism has not been fully elucidated. Our results showed that HQH increased the protein expressions of synaptopodin, podocin, WT-1, and Bcl-2, decreased the protein expressions of Bax and cleaved-casepase-3, and activated the NF-ĸB and PI3K/AKT/mTOR pathway in MPC5 cells exposed to high-glucose (HG). Real-time PCR results showed that HQH reduced the mRNA expression of TNF-α, IL-1β, MCP-1, and IL-6. In conclusion, our results showed that HQH may attenuate podocyte injury by inhibiting MPC5 cell apoptosis induced by HG and NF-κB-mediated inflammation response through activation of the PI3K/AKT/mTOR pathway.
糖尿病肾病（DKD）是糖尿病常见的微血管并发症之一，目前仍缺乏有效的治疗方法。槐杞黄（HQH）是一种中药复方制剂，主要由槐耳菌质、枸杞子和黄精制成。已有研究表明其对DKD的治疗有效，但具体机制尚未完全阐明。我们的结果显示，HQH可增加MPC5细胞中突触足蛋白、足突蛋白、WT-1和Bcl-2的蛋白表达，降低Bax和裂解型半胱天冬酶-3的蛋白表达，并激活高糖（HG）处理的MPC5细胞中的NF-κB和PI3K/AKT/mTOR信号通路。实时PCR结果表明，HQH可降低TNF-α、IL-1β、MCP-1和IL-6的mRNA表达。总之，我们的结果表明，HQH可能通过激活PI3K/AKT/mTOR信号通路，抑制HG诱导的MPC5细胞凋亡和NF-κB介导的炎症反应，从而减轻足细胞损伤。
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2021-1
World J Diabetes. 2025-6-15
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