Guangxi Key Laboratory of Special Biomedicine and Advanced Institute for Brain and Intelligence, School of Medicine, Guangxi University, Nanning 530004, China.
Department of Urology, PLA Naval Medical Center, Naval Medical University, Shanghai 200052, China.
Biomolecules. 2024 Aug 23;14(9):1044. doi: 10.3390/biom14091044.
Lower urinary tract symptoms (LUTS) are common in postmenopausal women. These symptoms are often linked to decreased estrogen levels following menopause. This study investigated the relationship between estrogen levels, alterations in bladder tissue structure, bladder function, and the incidence of urinary frequency. An age-appropriate bilateral ovariectomized mouse model (OVX) was developed to simulate conditions of estrogen deficiency. Mice were divided into three groups: a sham-operated control group, OVX, and an estradiol-treated group. The assessments included estrogen level measurement, urination frequency, cystometry, histological analysis, immunofluorescence staining, and real-time quantitative PCR. Additionally, we quantified the expression of the mechanosensitive channel proteins and in mouse bladder tissues. Lower estrogen levels were linked to increased voiding episodes and structural changes in mouse bladder tissues, notably a significant increase in fiber deposition. There was a detectable negative relationship between estrogen levels and the expression of and , mechanosensitive proteins in mouse bladder tissues, which may influence voiding frequency and nocturia. Estrogen treatment could improve bladder function, decrease urination frequency, and reduce collagen deposition in the bladder tissues. This study explored the connection between estrogen levels and urinary frequency, potentially setting the stage for novel methods to address frequent urination symptoms in postmenopausal women.
下尿路症状（LUTS）在绝经后妇女中很常见。这些症状通常与绝经后雌激素水平下降有关。本研究调查了雌激素水平、膀胱组织结构改变、膀胱功能和尿频发生率之间的关系。建立了一个适合年龄的双侧卵巢切除小鼠模型（OVX）来模拟雌激素缺乏的情况。将小鼠分为三组：假手术对照组、OVX 组和雌二醇处理组。评估包括雌激素水平测量、排尿频率、膀胱测压、组织学分析、免疫荧光染色和实时定量 PCR。此外，我们还定量了小鼠膀胱组织中机械敏感通道蛋白 和 的表达。较低的雌激素水平与小鼠膀胱组织中排尿次数增加和结构变化有关，特别是 纤维沉积明显增加。雌激素水平和小鼠膀胱组织中机械敏感蛋白 和 的表达之间存在可检测的负相关关系，这可能会影响排尿频率和夜尿症。雌激素治疗可以改善膀胱功能，减少排尿频率，并减少膀胱组织中的胶原沉积。本研究探讨了雌激素水平与尿频之间的联系，为解决绝经后妇女频繁排尿症状提供了新的方法。
