Yao Huan, Liu Kang, Peng Lei, Huang Touli, Shi Jinzhen, Sun Beilin, Zou Juan
College of Biological and Food Engineering, Huaihua University, Huaihua 418000, China.
Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua 418000, China.
Biology (Basel). 2024 Sep 17;13(9):730. doi: 10.3390/biology13090730.
, commonly known as Tian Ma, is a perennial mycoheterotrophic orchid. Qianyang Tian Ma (QTM), a geographical indication agricultural product from Hongjiang City, Hunan Province, China, is primarily characterized by the red variety, f. . A severe outbreak of tuber brown rot disease was documented in QTM during the harvesting season in Hunan. The fungal pathogen associated with the disease was isolated on potato saccharose agar (PSA) and identified through morphological and phylogenetic analyses. Pathogenicity tests were performed on healthy tubers of f. . The results showed that the representative isolate, named TMB, produced white hyphal colonies with a ring structure, broom-like phialides, partially curved ellipsoidal conidia, and orange-yellow spherical ascocarps on PSA. Phylogenetic analysis of the ITS, LSU, and sequences using Bayesian and maximum-likelihood methods identified the isolate TMB as , based on morphological and phylogenetic data. Pathogenicity tests revealed typical disease symptoms on healthy tubers 15 days post-inoculation with the isolate TMB. is known to cause diseases in economically important crops, but there are no reports of its occurrence on f. in China. This study provides valuable insights into the occurrence, prevention, and control of brown rot disease in f. .
，俗称天麻，是一种多年生菌根异养兰花。黔阳天麻（QTM）是中国湖南省洪江市的一种地理标志农产品，主要特征为红色品种，f. 。在湖南收获季节，QTM发生了严重的块茎褐腐病疫情。与该病相关的真菌病原体在马铃薯蔗糖琼脂（PSA）上分离，并通过形态学和系统发育分析进行鉴定。对f. 的健康块茎进行了致病性测试。结果表明，代表性分离株TMB在PSA上产生白色菌丝菌落，具环状结构、帚状瓶梗、部分弯曲的椭圆形分生孢子和橙黄色球形子囊果。使用贝叶斯和最大似然法对ITS、LSU和序列进行系统发育分析，根据形态学和系统发育数据将分离株TMB鉴定为。致病性测试显示，接种分离株TMB 15天后，健康块茎上出现典型病害症状。已知会在经济上重要的作物中引起疾病，但在中国尚未有其在f. 上发生的报道。本研究为f. 褐腐病的发生、预防和控制提供了有价值的见解。
