Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, China.
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, The Chinese Academy of Sciences, Changchun 130102, China.
Int J Mol Sci. 2024 Sep 21;25(18):10149. doi: 10.3390/ijms251810149.
, a halophytic perennial grass belonging to the Poaceae family, thrives in saline-alkali grasslands and harbors a rich repository of resistance-related genetic resources. This study focused on deciphering the stress-responsive mechanisms of by conducting transcriptomic sequencing under NaHCO stress, which resulted in the annotation of a segment corresponding to the 51WRKY gene. The alkali-induced gene (QIG37591) was identified by phylogenetic analysis. Real-time quantitative PCR analysis was performed on plants subjected to PEG6000 and alkaline salt (NaHCO) stress, and the results indicated that the gene was upregulated in both the leaves and roots. The localization of the LcWRKY40 protein was confirmed by the use of green fluorescent protein (GFP) fusion technology in transformed rice protoplast cells. The GAL4-driven transformation of the gene in INVScI yeast cells, which exhibited enhanced tolerance upon overexpression of the gene under mannitol and alkaline salt (NaHCO) stress conditions. Under drought stress using mannitol, the fresh weight of Nicotiana tabacum overexpressing the gene was significantly higher than that of wild-type(WT) tobacco. Through drought and salt alkali stress, we found that overexpressed tobacco at different stages always outperformed the wild type in terms of fresh weight, SOD, MDA, and Fv/Fm. This study provides preliminary insights into the involvement of the gene in responding to drought and alkaline salt stresses, highlighting its role in enhancing plant resistance to drought and saline-alkaline conditions. These findings lay the foundation for future molecular breeding strategies aimed at improving grass resistance from different aspects.
是禾本科的一种盐生多年生草本植物，生长在盐碱性草原中，蕴藏着丰富的抗性相关遗传资源。本研究通过对 NaHCO 胁迫下进行转录组测序，以解析 的胁迫响应机制，注释到一个与 51WRKY 基因相对应的片段。通过系统进化分析鉴定到碱诱导基因 （QIG37591）。对 PEG6000 和碱性盐（NaHCO）胁迫下的 植株进行实时定量 PCR 分析，结果表明该基因在叶片和根中均上调表达。利用绿色荧光蛋白（GFP）融合技术在转化的水稻原生质体细胞中证实了 LcWRKY40 蛋白的定位。通过 GAL4 驱动的 基因在 INVScI 酵母细胞中的转化，发现该基因在甘露醇和碱性盐（NaHCO）胁迫条件下过表达时，酵母的耐受力增强。在甘露醇模拟干旱胁迫下，过表达 基因的烟草的鲜重明显高于野生型（WT）烟草。通过干旱和盐碱性胁迫实验，我们发现过表达烟草在不同阶段的鲜重、SOD、MDA 和 Fv/Fm 总是优于野生型。本研究初步揭示了 基因参与响应干旱和碱性盐胁迫的机制，强调了其在增强植物对干旱和盐碱性条件的抗性中的作用。这些发现为未来从不同方面提高草类抗性的分子育种策略奠定了基础。
