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哈斯鳄梨(米勒)果皮提取物对厄瓜多尔影响科拉属物种的、和病原体具有抗菌和抗氧化特性。

Hass Avocado ( Mill) Peel Extract Reveals Antimicrobial and Antioxidant Properties against , , and Pathogens Affecting Colla Species, in Ecuador.

作者信息

Donoso Caterine, Raluca Mihai A, Chávez-Jinez Stephanie, Vera Edwin

机构信息

Departamento de Ciencias de Alimentos y Biotecnología, Facultad de Ingeniería Química y Agroindustria, Escuela Politécnica Nacional EPN, Quito 170143, Ecuador.

Army Scientific and Technological Research Center-CICTE, Department of Life Science and Agriculture, Universidad de Las Fuerzas Armadas-ESPE, Av. General Ruminahui s/n y, Sangolqui 171103, Ecuador.

出版信息

Microorganisms. 2024 Sep 23;12(9):1929. doi: 10.3390/microorganisms12091929.

Abstract

The utilization of agroindustrial residues, such as avocado peel, as a source of bioactive compounds with antioxidant properties has garnered significant attention. In this study, we investigated the antioxidant potential using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ORAC (oxygen radical absorbance capacity) methods, along with the antimicrobial activity of phenolic compounds extracted from Hass avocado peel. These soluble polyphenols were quantified and identified using high-performance liquid chromatography (HPLC). The research focused on their effects against three fungal pathogens, , , and , which significantly impact banana crops, an essential agricultural commodity in Ecuador. The results have revealed that the application of 80% ethanol as an organic solvent led to increased soluble polyphenol content compared to 96% ethanol. Extraction time significantly influenced the phenolic content, with the highest values obtained at 90 min. Interestingly, despite substantial mycelial growth observed across all extract concentrations, the antifungal effect varied among the pathogens. Specifically, exhibited the highest sensitivity, while and were less affected. These results underscore the importance of considering both antioxidant and antimicrobial properties when evaluating natural extracts for potential applications in plant disease management.

摘要

利用农业工业残渣,如鳄梨皮,作为具有抗氧化特性的生物活性化合物来源已引起广泛关注。在本研究中,我们使用DPPH(2,2-二苯基-1-苦基肼)和ORAC(氧自由基吸收能力)方法研究了从哈斯鳄梨皮中提取的酚类化合物的抗氧化潜力以及抗菌活性。这些可溶性多酚通过高效液相色谱(HPLC)进行定量和鉴定。该研究聚焦于它们对三种真菌病原体( 、 和 )的影响,这三种病原体对厄瓜多尔重要农产品香蕉作物有显著影响。结果表明,与96%乙醇相比,使用80%乙醇作为有机溶剂可提高可溶性多酚含量。提取时间对酚类含量有显著影响,在90分钟时获得最高值。有趣的是,尽管在所有提取物浓度下均观察到大量菌丝生长,但不同病原体的抗真菌效果有所不同。具体而言, 表现出最高敏感性,而 和 受影响较小。这些结果强调了在评估天然提取物在植物病害管理中的潜在应用时,同时考虑其抗氧化和抗菌特性的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a16/11434585/d9f05465222e/microorganisms-12-01929-g001.jpg

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