Clark Kerry L, Hartman Shirley
Department of Public Health, University of North Florida, 1 UNF Drive, Jacksonville, FL 32224, USA.
Mandarin Wellness Center, Jacksonville, FL 32257, USA.
Pathogens. 2024 Aug 28;13(9):727. doi: 10.3390/pathogens13090727.
Lyme disease is the most commonly reported vector-borne disease in the United States. constitute an additional zoonotic pathogen whose public health impact and diversity continue to emerge. Rapid, sensitive, and specific detection of these and other vector-borne pathogens remains challenging, especially for patients with persistent infections. This report describes an approach for DNA extraction and PCR testing for the detection of spp. and spp. from dry blood spot (DBS) specimens from human patients. The present study included extraction of DNA and PCR testing of DBS samples from 105 patients with poorly defined, chronic symptoms labeled as Lyme-Like Syndromic Illness (LLSI). spp. DNA was detected in 20/105 (19%) and spp. DNA was detected in 41/105 (39%) patients with LLSI. Neither group of organisms was detected in DBS samples from 42 healthy control subjects. spp. 16S-23S rRNA internal transcribed spacer sequences were highly similar to ones previously identified in yellow flies, lone star ticks, a human patient from Florida, mosquitoes in Europe, or and strains from honeybees. These human strains may represent new genetic strains or groups of human pathogenic species of . The 41 spp. gene sequences obtained from human patients suggested the presence of four different species, including , , and /-like strains. These results suggest that specific aspects of the DBS DNA extraction and PCR approach enabled the detection of spp. and spp. DNA from very small amounts of human whole blood from some patients, including specimens stored on filter paper for 17 years.
莱姆病是美国报告最多的媒介传播疾病。[此处原文缺失部分内容]构成了另一种人畜共患病原体，其对公共卫生的影响和多样性仍在不断显现。快速、灵敏且特异的检测这些及其他媒介传播病原体仍然具有挑战性，尤其是对于持续性感染患者。本报告描述了一种从人类患者干血斑（DBS）标本中提取DNA并进行PCR检测以检测[此处原文缺失部分内容]菌属和[此处原文缺失部分内容]菌属的方法。本研究包括从105例被标记为莱姆病样综合征（LLSI）、症状不明确的慢性患者的DBS样本中提取DNA并进行PCR检测。在105例LLSI患者中，20例（19%）检测到[此处原文缺失部分内容]菌属DNA，41例（39%）检测到[此处原文缺失部分内容]菌属DNA。42名健康对照受试者的DBS样本中均未检测到这两类生物体。[此处原文缺失部分内容]菌属的16S - 23S rRNA内部转录间隔序列与先前在黄蝇、孤星蜱、一名来自佛罗里达的人类患者、欧洲蚊子或蜜蜂的[此处原文缺失部分内容]菌株中鉴定出的序列高度相似。这些人类菌株可能代表[此处原文缺失部分内容]的新遗传菌株或人类致病物种组。从人类患者获得的41个[此处原文缺失部分内容]菌属基因序列表明存在四种不同的物种，包括[此处原文缺失部分内容]、[此处原文缺失部分内容]以及[此处原文缺失部分内容]样菌株。这些结果表明，DBS DNA提取和PCR方法的特定方面能够从一些患者极少量的人类全血中检测到[此处原文缺失部分内容]菌属和[此处原文缺失部分内容]菌属DNA，包括在滤纸上保存了17年的标本。
