Mengele Isaac Joseph, Akoko James Miser, Shirima Gabriel Mkilema, Bwatota Shedrack Festo, Motto Shabani Kiyabo, Hernandez-Castro Luis E, Komwihangilo Daniel Mushumbusi, Lyatuu Eliamoni, Bronsvoort Barend Mark de Clare, Cook Elizabeth Anne Jessie
Department of Global Health and Biomedical Sciences, School of Life Science and Bioengineering, The Nelson Mandela African Institution of Science and Technology (NM-AIST), Arusha P.O. Box 447, Tanzania.
Tanzania Veterinary Laboratory Agency (TVLA), Central Zone Laboratory, Dodoma P.O. Box 543, Tanzania.
Pathogens. 2024 Sep 21;13(9):815. doi: 10.3390/pathogens13090815.
Brucellosis is a zoonosis caused by bacteria of the genus , which results in economic losses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of species in smallholder dairy cattle in six regions of Tanzania from July 2019 to October 2020. Dairy cattle ( = 2048) were sampled from 1371 farms. DNA extracted from blood and vaginal swabs was tested for using qPCR targeting the IS711 gene and positives were tested for the alkB marker for and BMEI1172 marker for . The molecular prevalence was 3.5% (95% CI: 2.8-4.4) with the highest prevalence 8.1% (95% CI: 4.6-13.0) in Njombe region. was the predominant species detected (66.2%). Further studies are recommended to understand the source of and its implications for veterinary public health. Livestock keepers should be informed of the risks and biosecurity practices to reduce the introduction and control of . Cattle and small ruminant vaccination programs could be implemented to control brucellosis in high-risk populations in the country.
布鲁氏菌病是一种由布鲁氏菌属细菌引起的人畜共患病，会导致与牲畜相关的经济损失并威胁公众健康。2019年7月至2020年10月，在坦桑尼亚六个地区开展了一项横断面研究，以确定小农户奶牛群中布鲁氏菌的分子流行率。从1371个农场采集了2048头奶牛的样本。采用针对IS711基因的qPCR对血液和阴道拭子提取的DNA进行布鲁氏菌检测，对阳性样本采用alkB标记物检测羊种布鲁氏菌，采用BMEI1172标记物检测牛种布鲁氏菌。分子流行率为3.5%（95%置信区间：2.8 - 4.4），其中Njombe地区流行率最高，为8.1%（95%置信区间：4.6 - 13.0）。检测到的主要菌种为羊种布鲁氏菌（66.2%）。建议开展进一步研究，以了解羊种布鲁氏菌的来源及其对兽医公共卫生的影响。应告知牲畜饲养者相关风险及生物安全措施，以减少羊种布鲁氏菌的传入和控制。可实施牛和小反刍动物疫苗接种计划，以控制该国高危人群中的布鲁氏菌病。
