Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, 430023, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, MWR &CAS, Wuhan, 430070, China.
Fish Shellfish Immunol. 2024 Nov;154:109930. doi: 10.1016/j.fsi.2024.109930. Epub 2024 Sep 26.
Leader RNAs are viral small non-coding RNAs that has been proved to play important roles in viral replication. Snakehead vesiculovirus (SHVV) is an aquatic virus that has caused huge economic loss in Chinese snakehead fish aquaculture industry. It has been proved that SHVV would generate leader RNA during the process of infection, and leader RNA could interact with viral nucleoprotein to promote viral replication. In this study, we identified that leader RNA could also interact with cellular protein Cold Shock Domain containing E1 (CSDE1) and heterogeneous nuclear ribonucleoproteins A3 (hnRNP A3). Further investigation reveals that overexpression of CSDE1 and hnRNP A3 facilitated SHVV replication. Downregulation of CSDE1 and hnRNP A3 by siRNA inhibited SHVV replication. This study provided a new sight into understand the mechanism of SHVV replication, and a potential anti-SHVV target for drug research.
病毒 RNA 是一类具有重要生物学功能的小 RNA 分子，它们在病毒复制、基因表达调控等方面发挥着重要作用。蛇头鱼虹彩病毒(Snakehead vesiculovirus, SHVV)是一种水生病毒，已被证实会在感染过程中产生 leader RNA，并且 leader RNA 可以与病毒核蛋白相互作用，从而促进病毒的复制。在本研究中，我们发现 leader RNA 还可以与细胞蛋白冷休克结构域包含 E1（Cold Shock Domain containing E1，CSDE1）和异质核核糖核蛋白 A3（heterogeneous nuclear ribonucleoproteins A3，hnRNP A3）相互作用。进一步的研究表明，CSDE1 和 hnRNP A3 的过表达促进了 SHVV 的复制。通过 siRNA 下调 CSDE1 和 hnRNP A3 抑制了 SHVV 的复制。本研究为理解 SHVV 的复制机制提供了新的视角，并为药物研究提供了一个潜在的抗 SHVV 靶点。
