Ryazan State Medical University, Ministry of Health of the Russian Federation, Ryazan, Russia.
Bull Exp Biol Med. 2024 Sep;177(5):630-634. doi: 10.1007/s10517-024-06238-1. Epub 2024 Sep 30.
The mechanisms of regulation of the organic anion transporting polypeptide OATP1B3 by sex hormones were studied using HepG2 cells. Estradiol, progesterone, and testosterone were added to cells at concentrations of 1, 10, 100 μM for 24 h. The relative content of OATP1B3 was evaluated by Western blotting. Estradiol at concentrations of 10 and 100 μM increased the level of OATP1B3 acting through the farnesoid X-receptor, testosterone at concentrations of 1, 10, and 100 μM increased the expression of the transporter protein due to its effect on the liver X-receptor subtype α (LXRα), and progesterone did not affect the expression of OATP1B3.
采用 HepG2 细胞研究了性激素对有机阴离子转运多肽 OATP1B3 的调节机制。向细胞中加入浓度为 1、10、100 μM 的雌二醇、孕酮和睾酮 24 h。通过 Western blot 评估 OATP1B3 的相对含量。浓度为 10 和 100 μM 的雌二醇通过法尼醇 X 受体增加 OATP1B3 的水平，浓度为 1、10 和 100 μM 的睾酮由于其对肝 X 受体亚型 α (LXRα) 的作用增加了转运蛋白的表达，而孕酮不影响 OATP1B3 的表达。
