Department of Central Laboratory, Liaocheng People's Hospital, Liaocheng, P.R. China.
Department of Clinical Laboratory, Liaocheng Women & Children Hospital, Liaocheng, P.R. China.
Future Oncol. 2024;20(37):2993-3008. doi: 10.1080/14796694.2024.2405459. Epub 2024 Sep 30.
To delineate the RNA-5-methylcytosine (m5C) modification of breast cancer brain metastasis (BCBM). Methylated RNA immunoprecipitation next-generation sequencing (MeRIP-seq) was performed to obtain RNA-m5C patterns of BCBM. 1048 hypermethylation and 1866 hypomethylation m5C peaks were identified in BCBM compared with those in breast cancer. The most significant m5C hypermethylated genes included ENG, SHANK1, IGFN1, EVL and MMP9, whereas the most significant m5C hypomethylated genes included AREG, SAA2, TP53I11, KRT7 and LCN2. MeRIP-qPCR data were concordant with the corresponding MeRIP-seq results in terms of the observed m5C levels. Conjoint analysis identified 190 hyper-up genes characterized by concurrent m5C hypermethylation and up-regulation, alongside 284 hypo-down genes exhibiting both m5C hypomethylation and down-regulation. This study presents the first comprehensive analysis of RNA-m5C modification in BCBM.
为了描绘乳腺癌脑转移（BCBM）中的 RNA-5-甲基胞嘧啶（m5C）修饰。采用甲基化 RNA 免疫沉淀测序（MeRIP-seq）方法获取 BCBM 的 RNA-m5C 图谱。与乳腺癌相比，BCBM 中鉴定出 1048 个高甲基化和 1866 个低甲基化 m5C 峰。m5C 高甲基化基因中最显著的包括 ENG、SHANK1、IGFN1、EVL 和 MMP9，而 m5C 低甲基化基因中最显著的包括 AREG、SAA2、TP53I11、KRT7 和 LCN2。MeRIP-qPCR 数据与相应的 MeRIP-seq 结果在观察到的 m5C 水平上是一致的。联合分析鉴定出 190 个特征为 m5C 高甲基化和上调的高上调基因，以及 284 个特征为 m5C 低甲基化和下调的低下调基因。这项研究首次对 BCBM 中的 RNA-m5C 修饰进行了全面分析。
