School of Nursing, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University.
Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine;
J Vis Exp. 2024 Sep 13(211). doi: 10.3791/67159.
Retinopathy can be observed in most ocular diseases and the late complications of diabetes mellitus. The specific pigment epithelial cells and optic cells' damage and degeneration are the main features of retinopathy. Many traditional medicines have shown substantial clinical efficacy in treating retinopathy. How to obtain a retina quickly and completely is a key step in traditional medicine research for the treatment of retinopathy. In this study, we aim to provide a standardized and exercisable procedure for sampling of N-methyl-N-nitrosourea (MNU)-induced rat's retina damage and multi-index evaluation of pathological changes. Rats were injected intraperitoneally with 60 mg/kg MNU once to induce retina damage, and retina samples were obtained after 7 days. Additionally, we performed hematoxylin-eosin staining to assess retinal pathological changes. Determination of the apoptosis rate and apoptosis protein by TUNEL and Western blot. These standardized protocols for retinal sampling and evaluation of pathological changes are helpful in promoting the exploration of the mechanism of retinopathy and the discovery of novel and effective traditional herbs.
视网膜病变可见于大多数眼部疾病和糖尿病的晚期并发症。视网膜病变的主要特征是特定的色素上皮细胞和光感受器细胞的损伤和变性。许多传统药物在治疗视网膜病变方面显示出显著的临床疗效。如何快速、完整地获取视网膜是传统医学研究治疗视网膜病变的关键步骤。在这项研究中，我们旨在为 N-甲基-N-亚硝脲（MNU）诱导的大鼠视网膜损伤的取样和多指标病理变化评估提供标准化和可操作的程序。大鼠一次性腹腔注射 60mg/kg MNU 诱导视网膜损伤，7 天后取视网膜样本。此外，我们进行了苏木精-伊红染色以评估视网膜的病理变化。通过 TUNEL 和 Western blot 测定细胞凋亡率和凋亡蛋白。这些标准化的视网膜取样和病理变化评估方案有助于促进对视网膜病变机制的探索和发现新的、有效的传统草药。
