BACKGROUND: In this study, individuals with unilateral palatally shifted canines had cone beam computed tomography (CBCT) image palatally displaced canine (PDC) and non-PDC sides investigate the anatomy of their upper teeth adopting a "surface matching" approach. PROCEDURES: The CBCT scans of an overall of 28 people with unilateral PDC were chosen. Every single tooth in research was broken down and turned into a three-dimensional structure utilizing identical research programs, which made it possible to quantify the total length of the tooth root. RESULTS: Assessing the lateral incisor (LI) roots quantities and widths of the control category, non-PDC side, and PDC sides revealed statistically significant variations ( < 0.001). CONCLUSIONS: Compared to contra-lateral LIs, LIs next to PDCs possess small roots. In addition, patients with unilateral PDCs had structural abnormalities between their canines and LIs.