Lin Nan-Chin, Hsia Shih-Min, Vu Nguyen Thanh-Hien, Wang Tong-Hong, Sun Kuo-Ting, Chiu Kuo-Chou, Shih Yin-Hwa, Shieh Tzong-Ming
School of Dentistry, College of Dentistry, China Medical University, Taichung, Taiwan.
Department of Oral and Maxillofacial Surgery, Changhua Christian Hospital, Changhua, Taiwan.
J Dent Sci. 2024 Oct;19(4):2074-2081. doi: 10.1016/j.jds.2024.05.001. Epub 2024 May 26.
BACKGROUND/PURPOSE: The long non-coding RNA (lncRNA) () exhibits diverse and complicated functions in cancer progression. Despite reports suggesting both tumor-suppressive and oncogenic effects in various cancers, its specific role in head and neck squamous cell carcinoma (HNSCC) remains unclear. This study aimed to investigate the association between expression levels and survival outcomes in HNSCC patients.
Paired tissue samples of tumor and non-cancerous matching tissues (NCMT) from 92 HNSCC patients were collected. expression was analyzed using RT-qPCR. Clinical characteristics, treatment received, and survival rates of the patients were assessed to determine the correlation with expression and explore its association with alcohol, betel quid, and cigarette use. Additionally, we examined the effect of arecoline on expression in normal human oral keratinocytes (NHOK) and fibroblasts (NHOF).
The study revealed a significant downregulation of expression in oral cancer tissues compared to NCMT. Meanwhile, arecoline increased expression in NHOK and NHOF cells. However, patients with downregulated expression exhibited higher overall survival rates, particularly in those who did not receive chemotherapy or radiotherapy.
expression levels are associated with survival outcomes in HNSCC patients, with upregulated expression indicating a worse prognosis, suggesting this lncRNA might contribute to cancer aggressiveness, especially in the absence of active treatment. These findings indicate may serve as a potential prognostic biomarker in HNSCC, but further research is required to elucidate its role in cancer progression and its potential as a therapeutic target.
背景/目的：长链非编码RNA（lncRNA）（）在癌症进展中表现出多样且复杂的功能。尽管有报道表明其在多种癌症中具有肿瘤抑制和致癌作用，但其在头颈部鳞状细胞癌（HNSCC）中的具体作用仍不清楚。本研究旨在探讨HNSCC患者中表达水平与生存结果之间的关联。
收集了92例HNSCC患者的肿瘤组织和非癌匹配组织（NCMT）的配对组织样本。使用RT-qPCR分析表达情况。评估患者的临床特征、接受的治疗和生存率，以确定与表达的相关性，并探讨其与酒精、槟榔和吸烟的关联。此外，我们检测了槟榔碱对正常人口腔角质形成细胞（NHOK）和成纤维细胞（NHOF）中表达的影响。
研究显示，与NCMT相比，口腔癌组织中的表达显著下调。同时，槟榔碱增加了NHOK和NHOF细胞中的表达。然而，表达下调的患者总体生存率较高，尤其是那些未接受化疗或放疗的患者。
HNSCC患者的表达水平与生存结果相关，表达上调表明预后较差，提示这种lncRNA可能促成癌症侵袭性，特别是在没有积极治疗的情况下。这些发现表明可能作为HNSCC的潜在预后生物标志物，但需要进一步研究以阐明其在癌症进展中的作用及其作为治疗靶点的潜力。
