Department of Civil and Environmental Engineering, Faculty of Engineering, University of Miyazaki, Miyazaki 889-2192, Japan.
Department of Civil and Environmental Engineering, Faculty of Engineering, University of Miyazaki, Miyazaki 889-2192, Japan.
J Hazard Mater. 2024 Dec 5;480:135987. doi: 10.1016/j.jhazmat.2024.135987. Epub 2024 Sep 27.
Antimicrobial-resistant bacteria, selected by antimicrobial agent use in livestock, are emerging and their spread to crops from feces via composting represents a public health concern as they are ultimately transmitted to humans. In this study, we investigated Escherichia coli and other ampicillin (AMP)-resistant coliform spread conditions in field soil and dent corn, an agricultural crop, on a livestock-derived compost-applying farm. No AMP-resistant E. coli was detected in any samples of field soil and dent corn. In contrast, AMP-resistant and extended-spectrum β-lactam (ESBL) producing coliforms were consistently present in field soil and dent corn during the entire study period. In particular, extremely high AMP-resistant coliform levels were detected in dent corn stems and roots. AMP-resistant coliform detection in crops is pivotal and raises significant concerns regarding antimicrobial-resistant bacterial spread. Furthermore, AMP-resistant coliform isolate identification defined Enterobacter bugandensis and Enterobacter asburiae as the dominant species among AMP-resistant coliforms, both tested positive for ESBL production. This means that high concentrations of AMP-resistant coliforms are to be present on farms where crops are grown. However, we identified no common species among the AMP-resistant coliforms in the compost, field soil, and dent corn samples. Therefore, the initial hypothesis of the compost being the source of antimicrobial-resistant bacteria was not confirmed. Although their source remains unknown, a certain antimicrobial-resistant bacterial concentration could nonetheless be detected in the field.
抗菌药物在畜牧业中的使用选择出了抗药性细菌，而这些细菌通过粪便在堆肥过程中传播到农作物上，这引起了公众健康方面的关注，因为它们最终会传播给人类。在这项研究中，我们调查了在使用畜牧业堆肥的农场中，野外土壤和玉米中的氨苄青霉素（AMP）耐药大肠埃希菌和其他大肠埃希菌以及其他耐氨苄青霉素（AMP）的大肠埃希菌和其他大肠埃希菌以及其他耐氨苄青霉素（AMP）的大肠埃希菌和其他大肠埃希菌以及其他耐氨苄青霉素（AMP）的大肠埃希菌和其他大肠埃希菌以及其他耐氨苄青霉素（AMP）的大肠埃希菌和其他大肠埃希菌以及其他耐氨苄青霉素（AMP）的大肠埃希菌和其他大肠埃希菌的传播情况。在野外土壤和玉米的任何样本中均未检测到 AMP 耐药大肠杆菌。相比之下，在整个研究期间，AMP 耐药和产超广谱β-内酰胺酶（ESBL）的大肠埃希菌一直存在于野外土壤和玉米中。特别是，在玉米茎和根中检测到了极高的 AMP 耐药大肠埃希菌水平。在农作物中检测到 AMP 耐药大肠埃希菌至关重要，这引起了对抗菌药物耐药细菌传播的严重关注。此外，通过对 AMP 耐药大肠埃希菌分离株的鉴定，确定了阴沟肠杆菌和阿氏肠杆菌为 AMP 耐药大肠埃希菌中的主要种属，它们都对 ESBL 产生呈阳性反应。这意味着在种植农作物的农场中，AMP 耐药大肠埃希菌的浓度可能会很高。然而，我们在堆肥、野外土壤和玉米样本中均未发现 AMP 耐药大肠埃希菌的共同种属。因此，堆肥是抗菌药物耐药细菌来源的初始假设未得到证实。尽管其来源尚不清楚，但在野外仍可能检测到一定浓度的抗菌药物耐药细菌。
