Ti WenGeng, Liu MeiYan, Xie AnYu, Wang YueYao, Wu SiJin, Sheng QianYing, Lan Minbo
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Talanta. 2025 Jan 1;282:126955. doi: 10.1016/j.talanta.2024.126955. Epub 2024 Sep 27.
Glycosylation and phosphorylation of proteins represent crucial forms of post-translational modifications (PTMs), playing pivotal roles in various biological processes. Research indicates a strong correlation between the development of type 2 diabetes (T2D) and abnormal protein translation in the body. Therefore, studying glycosylation and phosphorylation at the molecular level can be used for monitoring disease progression and refining research methodologies. In this study, the material is modified and functionally engineered by utilizing graphene oxide (GO) as the substrate, and incorporating titanium ions (Ti) into chondroitin sulfate. The composite was successfully applied to the selective enrichment of glycopeptides and phosphopeptides by utilizing the bifunctionality of hydrophilic interaction chromatography and metal ion chelation chromatography. This approach allowed for the capture of 57 glycopeptides and 2 phosphopeptides from normal human serum, and 141 glycopeptides and 10 phosphopeptides from T2D serum, respectively. This approach effectively tackles the challenges of detecting low-abundance glycopeptides and phosphopeptides in complex environments, enabling the successful capture from serum samples. The design and application of this material provide new insights into the development of PTMs and their connection to the study of T2D diabetes.
蛋白质的糖基化和磷酸化是翻译后修饰（PTM）的关键形式，在各种生物过程中发挥着核心作用。研究表明，2型糖尿病（T2D）的发展与体内蛋白质翻译异常之间存在密切关联。因此，在分子水平上研究糖基化和磷酸化可用于监测疾病进展并完善研究方法。在本研究中，以氧化石墨烯（GO）为底物，将钛离子（Ti）掺入硫酸软骨素中，对材料进行修饰和功能改造。利用亲水相互作用色谱和金属离子螯合色谱的双功能性，该复合材料成功应用于糖肽和磷酸肽的选择性富集。该方法分别从正常人血清中捕获了57种糖肽和2种磷酸肽，从T2D血清中捕获了141种糖肽和10种磷酸肽。该方法有效应对了在复杂环境中检测低丰度糖肽和磷酸肽的挑战，实现了从血清样本中的成功捕获。这种材料的设计和应用为PTM的发展及其与T2D糖尿病研究的联系提供了新的见解。
