脂联素肽（ADPH 129 - 137）不是肥胖患者CD8 T细胞的靶抗原。

ADIPOPHILIN PEPTIDE (ADPH 129-137) IS NOT A TARGET ANTIGEN FOR CD8 T-CELLS IN PATIENTS WITH OBESITY.

作者信息

Asadipour M, Ataollahi M R, Shams K, Ali-Hassanzadeh M, Martinuzzi E, Kalantar K

机构信息

Shiraz University of Medical Sciences, School of Medicine, Department of Immunology, Shiraz.

Fasa University of Medical Sciences, School of Medicine, Department of Immunology, Fasa.

出版信息

Acta Endocrinol (Buchar). 2024 Jan-Mar;20(1):21-26. doi: 10.4183/aeb.2024.21. Epub 2024 Oct 3.

DOI:10.4183/aeb.2024.21

PMID:39372296

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11449246/

Abstract

CONTEXT

In obesity, the infiltration of leukocytes into adipose tissue seems to play a key role in the development of inflammation and insulin resistance. Over-expression of adipophilin (ADPH) in adipose tissue, a protein which regulates lipid droplet structure and formation, has been reported in some studies.

OBJECTIVE

To investigate the role of ADPH 129-137 as a target for CD8+ T-cells in PBMCs of patients with obesity.

SUBJECTS AND METHODS

PBMCs were obtained from 9 non-diabetic obese patients and 11 healthy subjects expressing the HLA-A0201 molecule. The ELISPOT assay used to monitor the presence of IFN-γ producing CD8+ T-cells against a HLA class I-restricted epitope derived from Adipophilin (ADPH 129-137) and two control peptides: Flu MP58-66 and Melan-A27-35.

RESULTS

The outcomes showed no significant difference between patient group and healthy donors in response to ADPH 129-137.

CONCLUSION

These results demonstrated that ADPH 129-137 peptide possibly does not act as an autoantigen in patients with obesity.

摘要

背景

在肥胖症中，白细胞浸润到脂肪组织似乎在炎症和胰岛素抵抗的发展中起关键作用。一些研究报道了脂肪组织中脂联素（ADPH）的过表达，脂联素是一种调节脂滴结构和形成的蛋白质。

目的

研究ADPH 129 - 137作为肥胖患者外周血单核细胞（PBMCs）中CD8 + T细胞靶点的作用。

对象与方法

从9名非糖尿病肥胖患者和11名表达HLA - A0201分子的健康受试者中获取PBMCs。采用酶联免疫斑点试验（ELISPOT）监测针对源自脂联素（ADPH 129 - 137）的HLA I类限制性表位以及两种对照肽（流感病毒基质蛋白MP58 - 66和黑色素瘤抗原A27 - 35）产生干扰素-γ的CD8 + T细胞的存在情况。