Norrasarn Jukkraphop, Kaewtong Chatthai, Wanno Banchob, Tuntulani Thawatchai, Pulpoka Buncha
Nanotechnology Research Unit and Supramolecular Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Maha sarakham, 44150, Thailand.
Supramolecular Chemistry Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
J Fluoresc. 2025 Jul;35(7):5883-5890. doi: 10.1007/s10895-024-03969-2. Epub 2024 Oct 7.
This study presents the development of a sensitive and selective gold ion (Au) sensor utilizing layer-by-layer (LbL) assembled thin films composed of polyethylenimine (PEI) and poly (acrylic acid) (PAA) conjugated with rhodamine (Rho). The first study revealed that the polymeric sensors (PAA-Rho) demonstrated significant selectivity and sensitivity in their colorimetric and fluorescence responses to Au compared to other metal ions. In their spirolactam form, the polymeric sensors were non-fluorescent but could selectively transform into the fluorescent ring-opened amide form upon interaction with Au ions, resulting in fluorescence enhancement and observable color changes. Common co-existing metal ions showed negligible interference in the detection of Au. The LbL sensor exhibited a linear increase in absorbance with the addition of bilayers, confirming successful film deposition. Surface morphology analysis using SEM, along with structural confirmation via ATR-FTIR and XRD, further validated the sensor's capability to detect cation. Results demonstrated that the LbL sensor exhibited selectivity for Au ions within the range 1 × 10 to 1 × 10 M. This approach offers an easily understandable and intrinsically sensitive means for detecting Au ions in both environmental and biological applications.
本研究介绍了一种灵敏且具选择性的金离子（Au）传感器的研制，该传感器利用由聚乙烯亚胺（PEI）和与罗丹明（Rho）共轭的聚丙烯酸（PAA）组成的层层（LbL）组装薄膜。第一项研究表明，与其他金属离子相比，聚合物传感器（PAA-Rho）在对Au的比色和荧光响应方面表现出显著的选择性和灵敏度。在其螺内酰胺形式下，聚合物传感器无荧光，但与Au离子相互作用时可选择性地转变为荧光开环酰胺形式，导致荧光增强和可观察到的颜色变化。常见的共存金属离子在Au的检测中显示出可忽略不计的干扰。随着双层膜的添加，LbL传感器的吸光度呈线性增加，证实了薄膜沉积成功。使用扫描电子显微镜（SEM）进行的表面形态分析，以及通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）和X射线衍射（XRD）进行的结构确认，进一步验证了该传感器检测阳离子的能力。结果表明，LbL传感器对1×10至1×10 M范围内的Au离子具有选择性。这种方法为在环境和生物应用中检测Au离子提供了一种易于理解且本质上灵敏的手段。
