Thysen B, Bloch E, Varma S K
J Toxicol Environ Health. 1985;16(6):763-9. doi: 10.1080/15287398509530787.
The present study was undertaken to evaluate the endocrinologic and spermatogenic effects of 2,4-toluenediamine (TDA) in the rat. Adult male rats were fed 0,0.01, and 0.03% TDA ad libitum for 10 wk. At the end of wk 10 and at 11 wk post TDA treatment, the animals were killed, and cauda epididymal sperm counts and reproductive organ weights were determined. Blood samples were obtained for analyses of testosterone and gonadotropins. Treatment with 0.03% TDA for 10 wk reduced the weight of the seminal vesicles and epididymides and reduced serum testosterone levels. Cauda epididymal sperm counts were decreased in animals treated with 0.03% TDA for 10 wk and in TDA-treated animals placed on normal diet for 11 wk. Serum luteinizing hormone (LH) concentrations were increased and weights of epididymides and testes were reduced in 0.03%-TDA-treated animals placed on normal diet for 11 wk. The results indicate that TDA exerts a toxic effect on spermatogenesis and appears to affect androgen action and production in the male rat. Since the males exhibited reduced cauda epididymal sperm counts 11 wk after 0.03% TDA treatment, it appears that TDA induced damage to the germinal components of the testes.
本研究旨在评估2,4-甲苯二胺（TDA）对大鼠内分泌和生精的影响。成年雄性大鼠自由采食含0%、0.01%和0.03%TDA的饲料，持续10周。在第10周结束时以及TDA处理后第11周，处死动物，测定附睾尾部精子计数和生殖器官重量。采集血样分析睾酮和促性腺激素。用0.03%TDA处理10周可降低精囊和附睾重量，并降低血清睾酮水平。用0.03%TDA处理10周的动物以及用TDA处理后改喂正常饲料11周的动物，其附睾尾部精子计数均减少。用0.03%TDA处理后改喂正常饲料11周的动物，血清黄体生成素（LH）浓度升高，附睾和睾丸重量减轻。结果表明，TDA对生精有毒性作用，似乎会影响雄性大鼠的雄激素作用和产生。由于在0.03%TDA处理11周后雄性大鼠附睾尾部精子计数减少，看来TDA对睾丸的生精成分造成了损害。
