Department of Chemistry, University of California, Irvine, California.
Department of Molecular Biology and Biochemistry, University of California, Irvine, California.
Curr Protoc. 2024 Oct;4(10):e70036. doi: 10.1002/cpz1.70036.
Three-dimensional (3D) printing promises a revolution in laboratory creativity by enabling rapid prototyping, broader availability of scientific apparatuses, and transformative scientific workflows. We believe all chemistry and biology laboratories should equip themselves with one or more 3D printers and a critical mass of scientists trained to operate them. This overview surveys the techniques, intricacies, and pitfalls associated with 3D printing of functional parts, including measurements, computer-aided design, slicing, limitations of 3D printing, troubleshooting, tips for tricky filaments, and 3D printer maintenance. A flow cells are essential tools in chemistry and biology laboratories, we discuss techniques relevant to the construction of watertight 3D-printed parts. Finally, we articulate a set of principles required for reporting 3D-printed innovations to improve the field's reproducibility and encourage iterative improvements by other scientists. Ideally, authors, peer reviewers, and editors will adopt these principles. We hope these protocols inspire a new generation of publications applying 3D printing in chemistry and biology-especially highly reproducible inventions with the requisite detail and associated documentation. Such reports will facilitate broad adoption and creative iteration of the most innovative designs, thus accelerating discovery in chemistry and biology. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.
三维（3D）打印通过实现快速原型制作、更广泛的科学仪器可用性以及变革性的科学工作流程，有望引发实验室创新革命。我们认为，所有化学和生物学实验室都应该配备一台或多台 3D 打印机和一大批经过培训能够操作它们的科学家。本综述调查了 3D 打印功能部件相关的技术、复杂性和陷阱，包括测量、计算机辅助设计、切片、3D 打印的局限性、故障排除、棘手线材的技巧以及 3D 打印机维护。流动池是化学和生物学实验室的重要工具，我们讨论了与构建防水 3D 打印部件相关的技术。最后，我们阐述了一套报告 3D 打印创新所需的原则，以提高该领域的可重复性并鼓励其他科学家进行迭代改进。理想情况下，作者、同行评审员和编辑将采用这些原则。我们希望这些方案能够激发新一代在化学和生物学中应用 3D 打印的出版物，尤其是具有必要细节和相关文档的高度可重复发明。这样的报告将促进最具创新性设计的广泛采用和创造性迭代，从而加速化学和生物学领域的发现。© 2024 作者。Wiley Periodicals LLC 出版的《当代协议》。
