Anhui University of Chinese Medicine, Hefei 230031, China.
The Geriatrics, The Second Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230061, China.
Int Immunopharmacol. 2024 Dec 25;143(Pt 1):113342. doi: 10.1016/j.intimp.2024.113342. Epub 2024 Oct 14.
It has been reported that the symptoms of rheumatoid arthritis (RA) can be ameliorated by acupuncture, an external treatment of traditional Chinese medicine. However, the immune mechanism underlying its action is elusive. Accordingly, this study investigated the role and mechanism of manual acupuncture (MA) in collagen-induced arthritis (CIA) in mice. The results demonstrated that MA or NaB treatment reduced Articular Index scores and right paw thickness and alleviated synovial inflammation and cartilage damage. MA or NaB treatment altered the content and relative abundance of short-chain fatty acids, particularly butyric and propionic acids, in feces. Additionally, MA or NaB treatment elevated SCD1, SREBP1, and GPX4 protein expression in synovial tissues and GSH-px contents in serum while decreasing ROS fluorescence intensity and MDA contents in peripheral blood. A linear correlation was found between the relative expression of SCD1 and SREBP1 in synovial tissues and the contents of propionic acids and butyric acids in feces, as well as between the contents of propionic acids and butyric acids. In summary, MA regulates butyric acids to inhibit ferroptosis, therefore suppressing inflammation in RA.
据报道，针灸作为一种中医外治法可以改善类风湿关节炎（RA）的症状。然而，其作用的免疫机制尚不清楚。因此，本研究探讨了手动针灸（MA）在胶原诱导性关节炎（CIA）小鼠中的作用和机制。结果表明，MA 或 NaB 治疗可降低关节炎指数评分和右后足厚度，缓解滑膜炎症和软骨损伤。MA 或 NaB 治疗改变了粪便中短链脂肪酸（尤其是丁酸和丙酸）的含量和相对丰度。此外，MA 或 NaB 治疗可提高滑膜组织中 SCD1、SREBP1 和 GPX4 蛋白的表达以及血清中 GSH-px 的含量，同时降低外周血中 ROS 荧光强度和 MDA 含量。在滑膜组织中 SCD1 和 SREBP1 的相对表达与粪便中丙酸和丁酸的含量之间，以及丙酸和丁酸的含量之间存在线性相关。综上所述，MA 通过调节丁酸来抑制铁死亡，从而抑制 RA 中的炎症。
