茄子潜伏病毒位于感染细胞的叶绿体和细胞核中。
Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells.
机构信息
Lab. Fitovirologia e Fisiopatologia, Centro de Pesquisa de Sanidade Vegetal, Instituto Biológico, São Paulo, CEP 04014-002, SP, Brazil.
Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València), Valencia, 46022, Spain.
出版信息
Virol J. 2024 Oct 15;21(1):254. doi: 10.1186/s12985-024-02530-8.
Viroids that belong to genera Avsunviroid and Pelamovirod (family Avsunviroidae) replicate and accumulate in the chloroplasts of infected cells. In this report, we confirmed by RNA in situ hybridization using digoxigenin-UTP-labelled riboprobes that the positive strands of eggplant latent viroid (ELVd), the only member of genus Elaviroid within the family Avsunviroidae, also accumulate in the chloroplasts of infected cells. However, comparison of ELVd in situ hybridization signals with those from bona fide chloroplastic and nuclear non-coding RNAs, such as chloroplast 5S rRNA and U1 small nuclear RNA, supports the notion that this viroid is also present in the nuclei of infected cells. These results suggest that the subcellular localization of viroids within the family Avsunviroidae may be more complex than previously assumed with dynamic presence in several compartments during the infectious cycle.
属于银杏潜隐病毒属和 Pelamoviroid 属（银杏病毒科）的类病毒在感染细胞的叶绿体中复制和积累。在本报告中，我们通过使用 digoxigenin-UTP 标记的 RNA 原位杂交证实，茄子潜隐病毒（ELVd）的正链，作为银杏病毒科内银杏潜隐病毒属的唯一成员，也在感染细胞的叶绿体中积累。然而，将 ELVd 的原位杂交信号与真正的叶绿体和核非编码 RNA（如叶绿体 5S rRNA 和 U1 小核 RNA）的信号进行比较，支持这样的观点，即这种类病毒也存在于感染细胞的核中。这些结果表明，银杏病毒科内类病毒的亚细胞定位可能比以前假设的更复杂，在感染周期中存在于几个隔室中。
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