Department of Cell Biology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang 421001, China.
Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang 421001, China.
Molecules. 2024 Sep 30;29(19):4645. doi: 10.3390/molecules29194645.
This study aimed to enhance the ultrasonic-assisted extraction (UAE) yield of seawater polysaccharides (APPs) and investigate its structural characteristics and bioactivities. The optimization of UAE achieved a maximum crude polysaccharides yield of 14.78%. The optimal extraction conditions were a liquid-solid ratio of 30.00 mL/g, extraction temperature of 81 °C, ultrasonic power at 92 W and extraction time at 30 min. After purification through cellulose DEAE-52 and Sephadex G-100 columns, two polysaccharide elutions (APP-1 and APP-2) were obtained. APP-2 had stronger antioxidant and immunoregulatory activities than APP-1, thus the characterization of APP-2 was conducted. APP-2 was an acidic polysaccharide consisting of rhamnose, glucose, mannose and glucuronic acid at a ratio of 1.00:24.21:7.63:1.53. It possessed a molecular weight of 72.48 kDa. Additionally, APP-2 had linear and irregular spherical particles and amorphous structures, which contained pyranoid polysaccharides with alpha/beta glycosidic bonds. These findings offered the foundation for APP-2 as an antioxidant and immunomodulator applied in the food, pharmaceutical and cosmetic industries.
本研究旨在提高海水多糖（APPs）的超声辅助提取（UAE）产量，并研究其结构特征和生物活性。通过 UAE 的优化，粗多糖的最大得率达到了 14.78%。最佳提取条件为液固比 30.00 mL/g、提取温度 81°C、超声功率 92 W 和提取时间 30 min。经过纤维素 DEAE-52 和 Sephadex G-100 柱的纯化，得到了两种多糖洗脱液（APP-1 和 APP-2）。APP-2 具有比 APP-1 更强的抗氧化和免疫调节活性，因此对 APP-2 进行了表征。APP-2 是一种酸性多糖，由鼠李糖、葡萄糖、甘露糖和葡萄糖醛酸组成，比例为 1.00:24.21:7.63:1.53。它的分子量为 72.48 kDa。此外，APP-2 具有线性和不规则球形颗粒以及无定形结构，其中包含吡喃糖多糖和α/β糖苷键。这些发现为 APP-2 作为抗氧化剂和免疫调节剂在食品、制药和化妆品行业的应用提供了基础。
