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核心技术专利：CN118964589B侵权必究
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超声辅助提取、特性分析及多糖抗氧化和免疫调节活性的优化。

Optimization of Ultrasonic-Assisted Extraction, Characterization and Antioxidant and Immunoregulatory Activities of Polysaccharides.

机构信息

Department of Cell Biology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang 421001, China.

Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang 421001, China.

出版信息

Molecules. 2024 Sep 30;29(19):4645. doi: 10.3390/molecules29194645.

DOI:10.3390/molecules29194645
PMID:39407575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11477882/
Abstract

This study aimed to enhance the ultrasonic-assisted extraction (UAE) yield of seawater polysaccharides (APPs) and investigate its structural characteristics and bioactivities. The optimization of UAE achieved a maximum crude polysaccharides yield of 14.78%. The optimal extraction conditions were a liquid-solid ratio of 30.00 mL/g, extraction temperature of 81 °C, ultrasonic power at 92 W and extraction time at 30 min. After purification through cellulose DEAE-52 and Sephadex G-100 columns, two polysaccharide elutions (APP-1 and APP-2) were obtained. APP-2 had stronger antioxidant and immunoregulatory activities than APP-1, thus the characterization of APP-2 was conducted. APP-2 was an acidic polysaccharide consisting of rhamnose, glucose, mannose and glucuronic acid at a ratio of 1.00:24.21:7.63:1.53. It possessed a molecular weight of 72.48 kDa. Additionally, APP-2 had linear and irregular spherical particles and amorphous structures, which contained pyranoid polysaccharides with alpha/beta glycosidic bonds. These findings offered the foundation for APP-2 as an antioxidant and immunomodulator applied in the food, pharmaceutical and cosmetic industries.

摘要

本研究旨在提高海水多糖（APPs）的超声辅助提取（UAE）产量，并研究其结构特征和生物活性。通过 UAE 的优化，粗多糖的最大得率达到了 14.78%。最佳提取条件为液固比 30.00 mL/g、提取温度 81°C、超声功率 92 W 和提取时间 30 min。经过纤维素 DEAE-52 和 Sephadex G-100 柱的纯化，得到了两种多糖洗脱液（APP-1 和 APP-2）。APP-2 具有比 APP-1 更强的抗氧化和免疫调节活性，因此对 APP-2 进行了表征。APP-2 是一种酸性多糖，由鼠李糖、葡萄糖、甘露糖和葡萄糖醛酸组成，比例为 1.00:24.21:7.63:1.53。它的分子量为 72.48 kDa。此外，APP-2 具有线性和不规则球形颗粒以及无定形结构，其中包含吡喃糖多糖和α/β糖苷键。这些发现为 APP-2 作为抗氧化剂和免疫调节剂在食品、制药和化妆品行业的应用提供了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/29f9fa776570/molecules-29-04645-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/2309e6ef27bd/molecules-29-04645-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/15d301d37070/molecules-29-04645-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/9e315b8d4c19/molecules-29-04645-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/4ad9cb48e928/molecules-29-04645-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/4f90430a272d/molecules-29-04645-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/2d56d554d185/molecules-29-04645-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/3f58070d0a8f/molecules-29-04645-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/29f9fa776570/molecules-29-04645-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/2309e6ef27bd/molecules-29-04645-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/15d301d37070/molecules-29-04645-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/9e315b8d4c19/molecules-29-04645-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/4ad9cb48e928/molecules-29-04645-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/4f90430a272d/molecules-29-04645-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/2d56d554d185/molecules-29-04645-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/3f58070d0a8f/molecules-29-04645-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df30/11477882/29f9fa776570/molecules-29-04645-g008.jpg

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引用本文的文献

[1]
Extraction, Characterization, Biological Properties, and X-Ray Fluorescence Analysis of Functional Polysaccharides Derived from .

Life (Basel). 2025-7-31

[2]
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[3]
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本文引用的文献

[1]
Extraction, structural features, and pharmacological effects of the polysaccharides from Porphyra yezoensis: A review.

Int J Biol Macromol. 2024-11

[2]
Ultrasound-assisted extraction and encapsulation of betalain from prickly pear: Process optimization, in-vitro digestive stability, and development of functional gummies.

Ultrason Sonochem. 2024-8

[3]
Ultrasonic-assisted extraction of a low molecular weight polysaccharide from Nostoc commune Vaucher and its structural characterization and immunomodulatory activity.

Ultrason Sonochem. 2024-8

[4]
The relationship between polysaccharide structure and its antioxidant activity needs to be systematically elucidated.

Int J Biol Macromol. 2024-6

[5]
Optimization of ultrasound-assisted extraction of Imperata cylindrica polysaccharides and evaluation of its anti-oxidant and amelioration of uric acid stimulated cell apoptosis.

Ultrason Sonochem. 2024-3

[6]
Purification and structural characterization of two polysaccharides with anti-inflammatory activities from Plumbago zeylanica L.

Int J Biol Macromol. 2024-3

[7]
Ultrasonic extraction of Moringa oleifera seeds polysaccharides: Optimization, purification, and anti-inflammatory activities.

Int J Biol Macromol. 2024-2

[8]
Structural Characterization and Immunomodulatory Activity of a Mannose-Rich Polysaccharide Isolated from H4-2.

J Agric Food Chem. 2023-12-13

[9]
Effect of seawater on the biomass composition of Spirulina produced at a pilot-scale.

N Biotechnol. 2023-12-25

[10]
The potential, challenges, and prospects of the genus Spirulina polysaccharides as future multipurpose biomacromolecules.

Int J Biol Macromol. 2023-12-31

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