Institute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan 063299, China.
Tangshan Key Laboratory of Rice Breeding, Tangshan 063299, China.
Int J Mol Sci. 2024 Sep 26;25(19):10376. doi: 10.3390/ijms251910376.
Soil salinity poses a serious threat to rice production. The salt tolerance of rice at the germination stage is one of the major determinants of stable stand establishment, which is very important for direct seeding in saline soil. The complexity and polygenic nature of salt tolerance have limited the efficiency of discovering and cloning key genes in rice. In this study, an RIL population with an ultra-high-density genetic map was employed to investigate the salt-tolerant genetic basis in rice, and a total of 20 QTLs were detected, including a major and stable QTL (). Subsequently, salt-specific DEGs from a comparative transcriptome analysis were overlaid onto annotated genes located on a stable QTL interval, and eight putative candidate genes were further identified. Finally, from the sequence alignment and variant analysis, was confirmed to be the most promising candidate gene for regulating salinity tolerance in rice. This study provides important information for elucidating the genetic and molecular basis of rice salt tolerance at the germination stage, and the genes detected here will be useful for improvements in rice salt tolerance.
土壤盐度对水稻生产构成严重威胁。水稻在发芽阶段的耐盐性是稳定苗立建的主要决定因素之一，这对于在盐渍土中直接播种非常重要。耐盐性的复杂性和多基因性质限制了在水稻中发现和克隆关键基因的效率。在这项研究中，利用一个具有超高密度遗传图谱的 RIL 群体来研究水稻的耐盐遗传基础，共检测到 20 个 QTL，包括一个主要且稳定的 QTL（）。随后，将比较转录组分析中的盐特异性 DEGs 叠加到稳定 QTL 区间上注释的基因上，进一步鉴定了 8 个候选基因。最后，通过序列比对和变异分析，确定 是调控水稻发芽阶段耐盐性的最有希望的候选基因。本研究为阐明水稻发芽阶段耐盐性的遗传和分子基础提供了重要信息，这里检测到的基因将有助于提高水稻的耐盐性。
