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27-羟胆固醇可负向影响骨髓内皮细胞在骨髓中的功能。

27-Hydroxycholesterol Negatively Affects the Function of Bone Marrow Endothelial Cells in the Bone Marrow.

机构信息

Department of Convergence Medicine, School of Medicine, Pusan National University, Yangsan 50612, Republic of Korea.

Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, 17177 Stockholm, Sweden.

出版信息

Int J Mol Sci. 2024 Sep 29;25(19):10517. doi: 10.3390/ijms251910517.

DOI:10.3390/ijms251910517
PMID:39408846
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11477443/
Abstract

Hematopoietic stem cells (HSCs) reside in specific microenvironments that facilitate their regulation through both internal mechanisms and external cues. Bone marrow endothelial cells (BMECs), which are found in one of these microenvironments, play a vital role in controlling the self-renewal and differentiation of HSCs during hematological stress. We previously showed that 27-hydroxycholesterol (27HC) administration of exogenous 27HC negatively affected the population of HSCs and progenitor cells by increasing the reactive oxygen species levels in the bone marrow. However, the effect of 27HC on BMECs is unclear. To determine the function of 27HC in BMECs, we employed magnetic-activated cell sorting to isolate CD31 BMECs and CD31 cells. We demonstrated the effect of 27HC on CD31 BMECs and HSCs. Treatment with exogenous 27HC led to a decrease in the number of BMECs and reduced the expression of adhesion molecules that are crucial for maintaining HSCs. Our results demonstrate that BMECs are sensitively affected by 27HC and are crucial for HSC survival.

摘要

造血干细胞 (HSCs) 存在于特定的微环境中，这些微环境通过内部机制和外部线索来促进它们的调节。骨髓内皮细胞 (BMECs) 是这些微环境之一中的一种，在造血应激期间控制 HSCs 的自我更新和分化方面发挥着至关重要的作用。我们之前的研究表明，外源性 27-羟胆固醇 (27HC) 的给药通过增加骨髓中的活性氧水平，对 HSCs 和祖细胞群体产生负面影响。然而，27HC 对 BMECs 的影响尚不清楚。为了确定 27HC 在 BMECs 中的功能，我们采用了磁激活细胞分选技术来分离 CD31 BMECs 和 CD31 细胞。我们证明了 27HC 对 CD31 BMECs 和 HSCs 的影响。外源性 27HC 的处理导致 BMECs 数量减少，并降低了维持 HSCs 所必需的粘附分子的表达。我们的结果表明，BMECs 对 27HC 敏感，并且对 HSC 的存活至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eab1/11477443/59ceb010b9ca/ijms-25-10517-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eab1/11477443/87eed0bbb8ea/ijms-25-10517-g002.jpg
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Nat Commun. 2023 Jul 25;14(1):4101. doi: 10.1038/s41467-023-39586-z.
2
Vascular cell-adhesion molecule 1 (VCAM-1) regulates JunB-mediated IL-8/CXCL1 expression and pathological neovascularization.血管细胞黏附分子 1（VCAM-1）调控 JunB 介导体细胞白细胞介素 8（IL-8）/CXC 趋化因子配体 1（CXCL1）表达和病理性血管新生。
Commun Biol. 2023 May 13;6(1):516. doi: 10.1038/s42003-023-04905-z.
3
Novel endogenous endoplasmic reticulum transmembrane protein SURF4 suppresses cell death by negatively regulating the STING-STAT6 axis in myeloid leukemia.
新型内源性内质网跨膜蛋白SURF4通过负向调节髓系白血病中的STING-STAT6轴来抑制细胞死亡。
Cancer Commun (Lond). 2023 Mar;43(3):395-399. doi: 10.1002/cac2.12390. Epub 2022 Nov 6.
4
Role of reactive oxygen species in regulating 27-hydroxycholesterol-induced apoptosis of hematopoietic progenitor cells and myeloid cell lines.活性氧在调节 27-羟胆固醇诱导造血祖细胞和髓系细胞系凋亡中的作用。
Cell Death Dis. 2022 Oct 31;13(10):916. doi: 10.1038/s41419-022-05360-0.
5
Insights Into Bone Marrow Niche Stability: An Adhesion and Metabolism Route.对骨髓生态位稳定性的见解：一条粘附与代谢途径。
Front Cell Dev Biol. 2022 Jan 18;9:798604. doi: 10.3389/fcell.2021.798604. eCollection 2021.
6
Trained Immunity-Promoting Nanobiologic Therapy Suppresses Tumor Growth and Potentiates Checkpoint Inhibition.免疫训练促进型纳米生物治疗抑制肿瘤生长并增强检查点抑制。
Cell. 2020 Oct 29;183(3):786-801.e19. doi: 10.1016/j.cell.2020.09.059.
7
Cellular and Molecular Mechanisms of Environmental Pollutants on Hematopoiesis.环境污染物对造血作用的细胞和分子机制。
Int J Mol Sci. 2020 Sep 23;21(19):6996. doi: 10.3390/ijms21196996.
8
Haematopoietic stem cell self-renewal in vivo and ex vivo.体内和体外造血干细胞的自我更新。
Nat Rev Genet. 2020 Sep;21(9):541-554. doi: 10.1038/s41576-020-0241-0. Epub 2020 May 28.
9
Bone marrow niches in haematological malignancies.血液恶性肿瘤中的骨髓龛。
Nat Rev Cancer. 2020 May;20(5):285-298. doi: 10.1038/s41568-020-0245-2. Epub 2020 Feb 28.
10
Chronic activation of endothelial MAPK disrupts hematopoiesis via NFKB dependent inflammatory stress reversible by SCGF.内皮细胞 MAPK 的慢性激活通过 NFKB 依赖的炎症应激破坏造血，可被 SCGF 逆转。
Nat Commun. 2020 Feb 3;11(1):666. doi: 10.1038/s41467-020-14478-8.