Fisheries College, Hunan Agricultural University, Changsha 410128, China.
Yuelushan Laboratory, Changsha 410128, China.
Int J Mol Sci. 2024 Sep 30;25(19):10566. doi: 10.3390/ijms251910566.
The hybrid F offspring of (♂) and (♀) exhibit heterosis in disease resistance and also show abnormal sex differentiation. To understand the mechanism behind gonadal differentiation in the hybrid F, we analyzed the transcriptomes of , , and the hybrid F; screened for genes related to gonad development in these samples; and measured their expression levels. Our results revealed that compared to either or , the gene expressions in most sub-pathways of the SNARE interactions in the vesicular transport pathway in the hypothalamus, pituitary, and gonadal tissues of their hybrid F offspring were significantly up-regulated. Furthermore, insufficient transcription of genes involved in oocyte meiosis may be the main reason for the insufficient reproductive ability of the hybrid F offspring. Through transcriptome screening, we identified key molecules involved in gonad development, including HSD3B7, HSD17B1, HSD17B3, HSD20B2, CYP17A2, CYP1B1, CYP2AA12, UGT2A1, UGT1A1, and FSHR, which showed significant differences in expression levels in the hypothalamus, pituitary, and gonads of these fish. Notably, the expression levels of UGT1A1 in the gonads of the hybrid F were significantly higher than those in and . These results provide a scientific basis for further research on the gonadal differentiation mechanism of hybrid F offspring.
（♂）和（♀）的杂交 F 代在抗病性方面表现出杂种优势，同时也表现出异常的性别分化。为了了解杂交 F 代性腺分化的机制，我们分析了 、 、和杂交 F 的转录组；在这些样本中筛选与性腺发育相关的基因；并测量它们的表达水平。我们的结果表明，与 或 相比，杂交 F 代下丘脑、垂体和性腺组织中囊泡运输途径中 SNARE 相互作用的大多数亚途径的基因表达显著上调。此外，卵母细胞减数分裂过程中涉及的基因转录不足可能是杂交 F 代生殖能力不足的主要原因。通过转录组筛选，我们鉴定了参与性腺发育的关键分子，包括 HSD3B7、HSD17B1、HSD17B3、HSD20B2、CYP17A2、CYP1B1、CYP2AA12、UGT2A1、UGT1A1 和 FSHR，它们在这些鱼类的下丘脑、垂体和性腺中的表达水平存在显著差异。值得注意的是，杂交 F 代性腺中 UGT1A1 的表达水平明显高于 和 。这些结果为进一步研究杂交 F 代性腺分化机制提供了科学依据。
