Ference Christopher M, Keith Lisa M
Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Hilo, HI 96720, USA.
Oak Ridge Institute for Science and Education, 1299 Bethel Valley Road, Oak Ridge, TN 37830, USA.
Plants (Basel). 2024 Sep 25;13(19):2687. doi: 10.3390/plants13192687.
Macadamia nuts are, economically, the second most important crop in the state of Hawai'i. A recent decline in yield and acreage has been attributed to insect damage and diseases such as Macadamia Quick Decline (MQD) caused by and . To develop an improved methodology for the diagnosis and treatment of MQD, investigations were undertaken to better understand the pathosystem of the disease. These investigations included sampling from multiple locations from sectioned trees utilizing two methods of tissue collection and isolations using two baiting techniques. The collection of tissue from the cambium and phloem of trees after scraping away the bark and in locations of recent or current sap exudation using a narrow diameter steel awl proved to be an efficient means for the molecular detection of the MQD pathogens from infected trees exhibiting MQD symptoms. In addition, a more efficient and cost-effective baiting method using apple puree was developed.
澳洲坚果在经济上是夏威夷州第二重要的作物。最近产量和种植面积的下降归因于昆虫损害以及诸如由[未提及具体病因]引起的澳洲坚果快速衰退病（MQD）等病害。为了开发一种改进的MQD诊断和治疗方法，开展了调查以更好地了解该病害的病理系统。这些调查包括从分段树木的多个位置采样，采用两种组织采集方法，并使用两种诱饵技术进行分离。用窄直径钢锥刮去树皮后，在近期或当前有树液渗出的位置从形成层和韧皮部采集组织，被证明是从表现出MQD症状的受感染树木中分子检测MQD病原体的有效方法。此外，还开发了一种使用苹果泥的更高效且具成本效益的诱饵方法。
