Chacón Ruy D, Sánchez-Llatas Christian J, Astolfi-Ferreira Claudete S, Raso Tânia Freitas, Piantino Ferreira Antonio J
Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, São Paulo 05508-900, Brazil.
Department of Genetics, Physiology, and Microbiology, Faculty of Biology, Complutense University of Madrid (UCM), 28040 Madrid, Spain.
Animals (Basel). 2024 Oct 5;14(19):2867. doi: 10.3390/ani14192867.
Marek's disease is caused by , commonly known as Marek's disease virus (MDV). This pathogen infects various bird species resulting in a range of clinical manifestations. The gene, which is crucial for oncogenesis, has been extensively studied, but molecular investigations of MDV in noncommercial South American birds are limited. This study detected MDV in backyard and ornamental birds from Brazil and Peru and characterized the gene. MDV was confirmed in all seven outbreaks examined. Three isoforms of (S-, , and L-) and two to seven proline repeat regions (PRRs) were detected among the sequenced strains. At the amino acid level, genetic profiles with low and high virulence potential were identified. Phylogenetic analysis grouped the sequences into three distinct clusters. Selection pressure analysis revealed 18 and 15 codons under positive and negative selection, respectively. The results demonstrate significant MDV diversity in the studied birds, with both high and low virulence potentials. This study highlights the importance of monitoring and characterizing circulating MDV in backyard and ornamental birds, as they can act as reservoirs for future epidemiological outbreaks.
马立克氏病由 引起，通常称为马立克氏病病毒（MDV）。这种病原体感染各种鸟类，导致一系列临床表现。对肿瘤发生至关重要的 基因已得到广泛研究，但对南美洲非商业性鸟类中MDV的分子研究有限。本研究在巴西和秘鲁的后院鸟类和观赏鸟类中检测到MDV，并对 基因进行了表征。在所检查的所有七次疫情中均确认了MDV。在测序菌株中检测到 （S-、 和L-）的三种异构体以及两到七个脯氨酸重复区域（PRR）。在氨基酸水平上，鉴定出具有低和高毒力潜力的遗传图谱。系统发育分析将序列分为三个不同的簇。选择压力分析分别揭示了18个和15个处于正选择和负选择下的密码子。结果表明，在所研究的鸟类中MDV具有显著的多样性，既有高毒力潜力，也有低毒力潜力。本研究强调了监测和表征后院鸟类和观赏鸟类中循环MDV的重要性，因为它们可能成为未来流行病学爆发的宿主。
