Vazquez-Avendaño José Roberto, Cortez-Romero César, Ambríz-García Demetrio Alonso, Rodríguez-Suástegui José Luis, Hernández-Pichardo José Ernesto, Navarro-Maldonado María Del Carmen
Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, México City 3855, México.
Department of Biology of Reproduction, Division of Biological and Health Sciences, Universidad Autónoma Metropolitana Unidad Iztapalapa, México City 09310, México.
Animals (Basel). 2024 Oct 8;14(19):2894. doi: 10.3390/ani14192894.
Somatic cell nuclear transfer (SCNT) has great potential for the replication of high-commercial-value animals, threatened wild species for conservation purposes, and transgenic animals for biomedical purposes. However, SCNT has a low success rate due to intrinsic factors of the technique itself, which leads to low rates of embryonic development and epigenetic alterations in cloned embryos. The objective of this study was to evaluate the effect of OF-UF on the intracellular concentrations of ROS and GSH and the development of cloned and parthenogenetic embryos. The results do not show a beneficial effect on the development of parthenogenetic and cloned embryos at concentrations of 0.5% OF-UF. Furthermore, at 1% OF-UF, an adverse effect was observed in cloned embryos at the blastocyst stage and 2% OF and UF in parthenogenetic embryos during the first divisions. Decreases in ROS and GSH levels were observed in the parthenogenetic blastocysts treated with 1% OF-UF, but not in the clones, in which a higher concentration of GSH and a similar concentration of ROS were observed. No effect of OF-UF was observed on embryonic development and redox balance in sheep embryos cloned via handmade cloning.
体细胞核移植（SCNT）在复制高商业价值动物、用于保护目的的濒危野生动物以及用于生物医学目的的转基因动物方面具有巨大潜力。然而，由于该技术本身的内在因素，SCNT成功率较低，这导致克隆胚胎的胚胎发育率低和表观遗传改变。本研究的目的是评估OF-UF对活性氧（ROS）和谷胱甘肽（GSH）细胞内浓度以及克隆胚胎和孤雌胚胎发育的影响。结果表明，在0.5%的OF-UF浓度下，对孤雌胚胎和克隆胚胎的发育没有有益影响。此外，在1%的OF-UF浓度下，观察到克隆胚胎在囊胚阶段有不良影响，而在孤雌胚胎的第一次分裂期间，2%的OF和UF有不良影响。在用1%的OF-UF处理的孤雌囊胚中观察到ROS和GSH水平降低，但在克隆胚胎中未观察到，在克隆胚胎中观察到较高浓度的GSH和相似浓度的ROS。未观察到OF-UF对通过手工克隆技术克隆的绵羊胚胎的胚胎发育和氧化还原平衡有影响。
