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从秘鲁玉米中分离出的植物病原体REC01菌株的基因组序列数据草案。

Draft genome sequence data of strain REC01, a phytopathogen isolated from a Peruvian maize.

作者信息

Estrada Richard, Aragón Liliana, Pérez Wendy E, Romero Yolanda, Martínez Gabriel, Garcia Karina, Cruz Juancarlos, Arbizu Carlos I

机构信息

Dirección de Desarrollo Tecnológico Agrario, Instituto Nacional de Innovación Agraria (INIA), Av. La Molina 1981, Lima 15024, Peru.

Facultad de Agronomía, Universidad Nacional Agraria La Molina (UNALM), Av. La Molina s/n, Lima 15024, Peru.

出版信息

Data Brief. 2024 Sep 23;57:110951. doi: 10.1016/j.dib.2024.110951. eCollection 2024 Dec.

Abstract

represents a major phytopathogenic threat to maize crops worldwide. In this study, we present genomic sequence data of a phytopathogen isolated from a maize stem that shows obvious signs of vascular rot. Using rigorous microbiological identification techniques, we correlated the disease symptoms observed in an affected maize region with the presence of the pathogen. Subsequently, the pathogen was cultured in a suitable fungal growth medium and extensive morphological characterization was performed. In addition, a pathogenicity test was carried out in a DCA model with three treatments and seven repetitions. De novo assembly from Illumina Novaseq 6000 sequencing yielded 456 contigs, which together constitute a 42.8 Mb genome assembly with a GC % content of 48.26. Subsequent comparative analyses were performed with other genomes available in the NCBI database.

摘要

对全球玉米作物构成了重大的植物病原威胁。在本研究中,我们展示了从一株表现出明显维管束腐烂迹象的玉米茎中分离出的一种植物病原体的基因组序列数据。我们使用严格的微生物鉴定技术,将在受影响玉米区域观察到的疾病症状与病原体的存在相关联。随后,将该病原体在合适的真菌生长培养基中培养,并进行了广泛的形态学特征分析。此外,在一个具有三种处理和七次重复的DCA模型中进行了致病性测试。通过Illumina Novaseq 6000测序进行的从头组装产生了456个重叠群,它们共同构成了一个42.8 Mb的基因组组装,GC含量为48.26%。随后与NCBI数据库中其他可用基因组进行了比较分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7578/11474212/9e5dd49be7b2/gr1.jpg

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