Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs), Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, China.
Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs), Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Fish Shellfish Immunol. 2024 Dec;155:109972. doi: 10.1016/j.fsi.2024.109972. Epub 2024 Oct 17.
The application of artificial micro-diet is an effective way to improve and standardize the quality of aquatic animal larvae. However, the widespread adoption of micro-diet faces a bottleneck due to the limited utilization capacity of the larvae. A 30-day feeding experiment was carried out to investigate the effect of dietary succinic acid (SA) on the growth performance, digestive ability, intestinal development, and immunity of large yellow croaker larvae (initial body weight 11.33 ± 0.57 mg). Four isonitrogenous and isolipidic diets were formulated, incorporating 0.00 %, 0.01 %, 0.02 % and 0.03 % SA separately. The results showed that a diet with 0.02 % SA significantly increased both the final body weight and the specific growth rate of the larvae. Regarding digestive ability, 0.01 % SA supplementation significantly enhanced trypsin activity in both intestinal and pancreatic segments. In addition, 0.01 % SA supplementation notably improved amylase activity in the intestinal segment, while diets with 0.01%-0.02 % SA significantly improved lipase activity in the pancreatic segment. In terms of intestinal development, 0.01 % SA supplementation remarkably boosted the activities of alkaline-phosphatase and leucine-aminopeptidase on brush border membrane in intestine. Furthermore, 0.03 % SA supplementation significantly increased the expression of occludin. In terms of immunity, larvae fed diets with 0.01%-0.02 % SA exhibited significantly higher lysozyme activity compared to the control group. Supplementation with 0.01 % SA also significantly increased both iNOS activity and NO content. In summary, the findings of this study suggested that supplementing 0.02 % SA can improve the growth performance of large yellow croaker larvae by improving digestive enzymes activities, promoting intestinal development, and enhancing nonspecific immunity.
人工微饲料的应用是提高和规范水生动物幼体质量的有效方法。然而，由于幼体的利用能力有限，微饲料的广泛应用面临瓶颈。进行了为期 30 天的饲养实验，以研究饲料琥珀酸（SA）对大黄鱼幼体（初始体重 11.33±0.57mg）生长性能、消化能力、肠道发育和免疫力的影响。配制了四种等氮等脂的饲料，分别添加 0.00%、0.01%、0.02%和 0.03%的 SA。结果表明，含 0.02%SA 的饲料显著提高了幼体的终体重和特定生长率。在消化能力方面，0.01%SA 补充剂显著提高了肠段和胰段的胰蛋白酶活性。此外，0.01%SA 补充剂显著提高了肠段的淀粉酶活性，而 0.01%-0.02%SA 的饮食显著提高了胰段的脂肪酶活性。在肠道发育方面，0.01%SA 补充剂显著提高了肠刷状缘膜碱性磷酸酶和亮氨酸氨肽酶的活性。此外，0.03%SA 补充剂显著增加了 occludin 的表达。在免疫力方面，与对照组相比，摄食 0.01%-0.02%SA 饮食的幼鱼溶菌酶活性显著提高。0.01%SA 补充剂还显著提高了 iNOS 活性和 NO 含量。综上所述，本研究结果表明，添加 0.02%SA 可以通过提高消化酶活性、促进肠道发育和增强非特异性免疫力来提高大黄鱼幼体的生长性能。
