Forget G, Lacroix M J, Brown R C, Evans P H, Sirois P
Environ Res. 1986 Feb;39(1):124-35. doi: 10.1016/s0013-9351(86)80014-7.
The effect of glass fibers on rat alveolar macrophages was studied with a new perifusion technique which allows the sequential determination of cell-derived inflammatory mediators as well as estimation of cell viability and aggregation at the end of the incubation period. Our results showed that glass fibers induced dose-dependent release of prostaglandins and beta-glucuronidase from macrophages and the aggregation and death of these cells. These deleterious effects were clearly related to the length of the fibers, with the longer fibers (greater than or equal to 4-5 micron) being more active than the shorter ones (less than 3 micron). Furthermore, a short exposure of 1 hr followed by an 18-hr perifusion induced the same inflammatory and toxic effects on the macrophages as did leaving the fibers undisturbed for the complete 18-hr perifusion. It is concluded that glass fibers produce effects in cultures of rat alveolar macrophages qualitatively similar to those of asbestos, and that fiber length appears to be a critical determinant of toxicity.
采用一种新的灌流技术研究了玻璃纤维对大鼠肺泡巨噬细胞的影响，该技术可在孵育期结束时依次测定细胞衍生的炎症介质，并评估细胞活力和聚集情况。我们的结果表明，玻璃纤维可诱导巨噬细胞中前列腺素和β-葡萄糖醛酸酶的剂量依赖性释放以及这些细胞的聚集和死亡。这些有害影响与纤维长度明显相关，较长的纤维（大于或等于4 - 5微米）比较短的纤维（小于3微米）更具活性。此外，1小时的短时间暴露后再进行18小时的灌流，对巨噬细胞产生的炎症和毒性作用与将纤维在整个18小时灌流期间保持原状时相同。结论是，玻璃纤维在大鼠肺泡巨噬细胞培养物中产生的影响在性质上与石棉相似，并且纤维长度似乎是毒性的关键决定因素。
