Vandekerckhove J, Bugaisky G, Buckingham M
J Biol Chem. 1986 Feb 5;261(4):1838-43.
A procedure was developed to determine the percentage of skeletal muscle actin and cardiac actin present in different striated muscle tissues. The method was applied to 2 mg of actin mixtures isolated from various origins. All samples show simultaneous expression of both striated muscle isoactins, with the cardiac actin being the major form (congruent to 80%) in 11-day-old chick embryonic leg muscle, decreasing to approximately 50% values in the late fetal stage of chicken, mouse, and in fused mouse muscle cell cultures and becoming the minor species (less than 5%) in adult skeletal muscle tissues. We also find a significant amount (up to 20%) of the skeletal muscle isoform in adult heart (ventricle) of porcine, bovine, and human origin and no differences in muscle actin ratios in human atrium and ventriculum cells. Similarly, no significant variation in the actin ratios was observed between a normal heart and a heart from a patient with hereditary obstructive myopathy. For those cells and tissues where comparison with levels of mRNA was possible we mostly find a good correlation between the relative ratios of expression of cardiac and skeletal actin proteins and mRNAs.
已开发出一种程序来测定不同横纹肌组织中骨骼肌肌动蛋白和心肌肌动蛋白的百分比。该方法应用于从各种来源分离的2毫克肌动蛋白混合物。所有样本均显示两种横纹肌同工肌动蛋白同时表达，在11日龄鸡胚腿部肌肉中，心肌肌动蛋白为主要形式（约占80%），在鸡、小鼠的胎儿后期以及融合的小鼠肌肉细胞培养物中降至约50%，在成年骨骼肌组织中成为次要形式（低于5%）。我们还发现在猪、牛和人的成年心脏（心室）中有大量（高达20%）的骨骼肌同工型，并且在人的心房和心室细胞中肌肉肌动蛋白比例没有差异。同样，在正常心脏和患有遗传性阻塞性肌病患者的心脏之间，未观察到肌动蛋白比例有显著变化。对于那些能够与mRNA水平进行比较的细胞和组织，我们大多发现心肌和骨骼肌肌动蛋白蛋白质与mRNA表达的相对比例之间具有良好的相关性。
