• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

化疗点击：受体控制和生物正交趋化因子连接用于耐药性白血病 B 细胞的实时成像。

Chemo-Click: Receptor-Controlled and Bioorthogonal Chemokine Ligation for Real-Time Imaging of Drug-Resistant Leukemic B Cells.

机构信息

Centre for Inflammation Research, The University of Edinburgh, Edinburgh EH16 4UU, U.K.

IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, Edinburgh EH16 4UU, U.K.

出版信息

J Am Chem Soc. 2024 Nov 6;146(44):30565-30572. doi: 10.1021/jacs.4c12035. Epub 2024 Oct 23.

DOI:10.1021/jacs.4c12035
PMID:39441736
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11544607/
Abstract

Drug resistance in B cell leukemia is characterized by the coexpression of CXCR5 and CXCR3 chemokine receptors, making it a valuable biomarker for patient stratification. Herein, we report a novel platform of activatable chemokines to selectively image drug-resistant leukemic B cells for the first time. The C-terminal derivatization of the human chemokines CXCL13 and CXCL10 with bioorthogonal tetrazine-BODIPY and BCN groups retained binding and internalization via their cognate CXCR5 and CXCR3 receptors and enabled rapid fluorescence labeling of CXCR5+ CXCR3+ resistant B cells─but not drug-susceptible leukemic cells─via intracellular chemokine ligation. This modular chemical approach offers a versatile strategy for real-time immunophenotyping of cell populations with distinct chemokine profiles and will accelerate the design of new precision medicine tools to advance personalized therapies in blood tumors.

摘要

B 细胞白血病的耐药性表现为 CXCR5 和 CXCR3 趋化因子受体的共表达，使其成为患者分层的有价值的生物标志物。在此，我们首次报道了一种新型的趋化因子激活平台，可选择性地对耐药性白血病 B 细胞进行成像。通过生物正交四嗪-BODIPY 和 BCN 基团对人趋化因子 CXCL13 和 CXCL10 的 C 末端进行衍生化，保留了与它们同源的 CXCR5 和 CXCR3 受体的结合和内化，并通过细胞内趋化因子连接快速标记 CXCR5+CXCR3+耐药 B 细胞——而不是耐药性白血病细胞。这种模块化的化学方法为具有不同趋化因子特征的细胞群的实时免疫表型分析提供了一种通用策略，并将加速新的精准医学工具的设计，以推进血液肿瘤的个性化治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/604c2cae8029/ja4c12035_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/ea25f2ee081c/ja4c12035_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/ecf4373002fe/ja4c12035_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/85e4a64dcd65/ja4c12035_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/604c2cae8029/ja4c12035_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/ea25f2ee081c/ja4c12035_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/ecf4373002fe/ja4c12035_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/85e4a64dcd65/ja4c12035_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5f2/11544607/604c2cae8029/ja4c12035_0004.jpg

相似文献

1
Chemo-Click: Receptor-Controlled and Bioorthogonal Chemokine Ligation for Real-Time Imaging of Drug-Resistant Leukemic B Cells.化疗点击：受体控制和生物正交趋化因子连接用于耐药性白血病 B 细胞的实时成像。
J Am Chem Soc. 2024 Nov 6;146(44):30565-30572. doi: 10.1021/jacs.4c12035. Epub 2024 Oct 23.
2
Human B cell-attracting chemokine 1 (BCA-1; CXCL13) is an agonist for the human CXCR3 receptor.人B细胞趋化因子1（BCA-1；CXCL13）是人类CXCR3受体的激动剂。
Cytokine. 2001 Aug 7;15(3):113-21. doi: 10.1006/cyto.2001.0923.
3
Association of the CXCL9-CXCR3 and CXCL13-CXCR5 axes with B-cell trafficking in giant cell arteritis and polymyalgia rheumatica.CXCL9-CXCR3和CXCL13-CXCR5轴与巨细胞动脉炎和风湿性多肌痛中B细胞迁移的关联
J Autoimmun. 2021 Sep;123:102684. doi: 10.1016/j.jaut.2021.102684. Epub 2021 Jul 6.
4
Intracellular bioorthogonal labeling of glucagon receptor via tetrazine ligation.通过点击化学将胰高血糖素受体进行细胞内生物正交标记。
Bioorg Med Chem. 2021 Aug 1;43:116256. doi: 10.1016/j.bmc.2021.116256. Epub 2021 Jun 6.
5
Chemokines and relapses in childhood acute lymphoblastic leukemia: A role in migration and in resistance to antileukemic drugs.趋化因子与儿童急性淋巴细胞白血病的复发：在迁移及抗白血病药物耐药中的作用
Blood Cells Mol Dis. 2015 Oct;55(3):220-7. doi: 10.1016/j.bcmd.2015.07.001. Epub 2015 Jul 2.
6
Human osteoblasts express functional CXC chemokine receptors 3 and 5: activation by their ligands, CXCL10 and CXCL13, significantly induces alkaline phosphatase and beta-N-acetylhexosaminidase release.人成骨细胞表达功能性CXC趋化因子受体3和5：其配体CXCL10和CXCL13对其激活可显著诱导碱性磷酸酶和β-N-乙酰己糖胺酶释放。
J Cell Physiol. 2003 Jan;194(1):71-9. doi: 10.1002/jcp.10188.
7
Differential expression of chemokine receptors on peripheral blood B cells from patients with rheumatoid arthritis and systemic lupus erythematosus.类风湿关节炎和系统性红斑狼疮患者外周血B细胞趋化因子受体的差异表达
Arthritis Res Ther. 2005;7(5):R1001-13. doi: 10.1186/ar1776. Epub 2005 Jun 22.
8
PEG10 activation by co-stimulation of CXCR5 and CCR7 essentially contributes to resistance to apoptosis in CD19+CD34+ B cells from patients with B cell lineage acute and chronic lymphocytic leukemia.通过CXCR5和CCR7共刺激激活PEG10对B细胞系急性和慢性淋巴细胞白血病患者的CD19+CD34+B细胞抗凋亡起着重要作用。
Cell Mol Immunol. 2004 Aug;1(4):280-94.
9
CXC chemokine ligand 13 and CC chemokine ligand 19 cooperatively render resistance to apoptosis in B cell lineage acute and chronic lymphocytic leukemia CD23+CD5+ B cells.CXC趋化因子配体13和CC趋化因子配体19协同赋予B细胞系急性和慢性淋巴细胞白血病CD23+CD5+B细胞抗凋亡能力。
J Immunol. 2006 Nov 15;177(10):6713-22. doi: 10.4049/jimmunol.177.10.6713.
10
Selectively frequent expression of CXCR5 enhances resistance to apoptosis in CD8(+)CD34(+) T cells from patients with T-cell-lineage acute lymphocytic leukemia.CXCR5的选择性频繁表达增强了T细胞系急性淋巴细胞白血病患者CD8(+)CD34(+) T细胞对凋亡的抗性。
Oncogene. 2005 Jan 20;24(4):573-84. doi: 10.1038/sj.onc.1208184.

本文引用的文献

1
Dual-Locked Enzyme-Activatable Bioorthogonal Fluorescence Turn-On Imaging of Senescent Cancer Cells.双锁酶激活型生物正交荧光开启成像检测衰老癌细胞。
J Am Chem Soc. 2024 Aug 14;146(32):22689-22698. doi: 10.1021/jacs.4c07286. Epub 2024 Aug 5.
2
On-Demand Thio-Succinimide Hydrolysis for the Assembly of Stable Protein-Protein Conjugates.按需硫代琥珀酰亚胺水解法用于稳定的蛋白质-蛋白质偶联物的组装。
J Am Chem Soc. 2024 Jul 31;146(30):20709-20719. doi: 10.1021/jacs.4c03721. Epub 2024 Jul 16.
3
Development of a Mature B Lymphocyte Probe through Gating-Oriented Live-Cell Distinction (GOLD) and Selective Imaging of Topical Spleen.
通过门控导向的活细胞区分（GOLD）和局部脾脏的选择性成像开发成熟B淋巴细胞探针。
JACS Au. 2024 Feb 26;4(4):1450-1457. doi: 10.1021/jacsau.4c00001. eCollection 2024 Apr 22.
4
Novel Fluorescent Strategy for Discriminating T and B Lymphocytes Using Transport System.利用转运系统区分T淋巴细胞和B淋巴细胞的新型荧光策略
Pharmaceutics. 2024 Mar 19;16(3):424. doi: 10.3390/pharmaceutics16030424.
5
Inserting "OFF-to-ON" BODIPY Tags into Cytokines: A Fluorogenic Interleukin IL-33 for Real-Time Imaging of Immune Cells.将“关到开”型硼二吡咯（BODIPY）标签插入细胞因子：用于免疫细胞实时成像的荧光白细胞介素IL-33
ACS Cent Sci. 2023 Dec 20;10(1):143-154. doi: 10.1021/acscentsci.3c01125. eCollection 2024 Jan 24.
6
Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles.用于成像Polo样激酶4和中心粒的化学探针。
JACS Au. 2023 Aug 4;3(8):2247-2256. doi: 10.1021/jacsau.3c00271. eCollection 2023 Aug 28.
7
Bioorthogonal Caging-Group-Free Photoactivatable Probes for Minimal-Linkage-Error Nanoscopy.用于最小连接误差纳米显微镜的无生物正交笼形基团光可激活探针。
ACS Cent Sci. 2023 Jul 26;9(8):1581-1590. doi: 10.1021/acscentsci.3c00746. eCollection 2023 Aug 23.
8
An AND-gate bioluminescent probe for precise tumor imaging.一种用于精确肿瘤成像的与门生物发光探针。
Chem Sci. 2023 May 3;14(21):5768-5773. doi: 10.1039/d3sc00556a. eCollection 2023 May 31.
9
Dual-locked spectroscopic probes for sensing and therapy.用于传感与治疗的双锁光谱探针。
Nat Rev Chem. 2021 Jun;5(6):406-421. doi: 10.1038/s41570-021-00277-2. Epub 2021 May 20.
10
Acid-Resistant BODIPY Amino Acids for Peptide-Based Fluorescence Imaging of GPR54 Receptors in Pancreatic Islets.用于基于肽的胰腺胰岛 GPR54 受体荧光成像的耐酸 BODIPY 氨基酸
Angew Chem Int Ed Engl. 2023 May 8;62(20):e202302688. doi: 10.1002/anie.202302688. Epub 2023 Apr 12.