Li Yaojun, Chen Shanmiao, Liu Shoulei
Department of Urology Surgery, Tongxiang First People's Hospital, Tongxiang, 314500, Zhejiang, China.
Cell Biochem Biophys. 2025 Jun;83(2):1593-1604. doi: 10.1007/s12013-024-01569-2. Epub 2024 Oct 25.
It was to explore the immune outcome of co-culture of dendritic cells (DC) and cytokine-induced killer cells (CIK) on prostate cancer (PCa) cells. Peripheral blood mononuclear cells (PBMCs) were extracted from healthy blood donors. DC and CIK cells were induced and co-cultured. The proliferation and phenotypic changes of DC, CIK, and DC-CIK cells/groups were observed. Model rats were constructed by injecting PC3 PCa cells into the abdominal cavity. The successful 50 cases were divided into a negative control group, a chemotherapy group, a DC group, a CIK group, and a DC-CIK treatment group (each consisting of 10 rats) to observe tumor progression. The secreted concentrations of interleukin-12 (IL-12) ((103.67 ± 2.77) pg/mL) and interferon-γ (IFN-γ) ((730.09 ± 23.52) pg/mL) were higher in DC-CIK group as against DC and CIK groups; the proliferation of CIK was higher in DC-CIK group as against CIK within 12 to 20 days of culture. The positive rate (PR) of CD3/ CD56 and CD8 was higher and that of CD45RA was lower in DC-CIK group as against CIK.The killing rate of the DC-CIK group was higher than that of the DC and CIK groups at a target effect ratio of 10:1/20:1/50:1 (P < 0.05). After the treatment, the body weight of rats in the chemotherapy group, DC group, and CIK group was significantly reduced (P < 0.05), while no significant changes were observed in the negative control group and DC-CIK group (P > 0.05). After 25 days of treatment, the tumor size in the DC-CIK group was significantly smaller compared to the negative control group, chemotherapy group, DC group, and CIK group; the necrotic area of the tumor tissue in the DC-CIK group was also significantly larger than that in the negative control group, chemotherapy group, DC group, and CIK group (P < 0.05). Co-culture of DC and CIK is excellent in enhancing the proliferation of CIK cells, increasing the secretion of IL-12 and IFN-γ, and enhancing the activity of immune cells and anti-tumor ability, showing its potential in anti-PCa tumor immunotherapy.
旨在探讨树突状细胞（DC）与细胞因子诱导的杀伤细胞（CIK）共培养对前列腺癌细胞（PCa）的免疫效应。从健康献血者中提取外周血单个核细胞（PBMC）。诱导并共培养DC和CIK细胞。观察DC、CIK及DC-CIK细胞/组的增殖及表型变化。通过将PC3前列腺癌细胞注入腹腔构建模型大鼠。将成功建模的50只大鼠分为阴性对照组、化疗组、DC组、CIK组和DC-CIK治疗组（每组10只大鼠），观察肿瘤进展情况。DC-CIK组白细胞介素-12（IL-12）（（103.67±2.77）pg/mL）和干扰素-γ（IFN-γ）（（730.09±23.52）pg/mL）的分泌浓度高于DC组和CIK组；培养12至20天时，DC-CIK组CIK的增殖高于CIK组。与CIK组相比，DC-CIK组CD3/CD56和CD8的阳性率较高，CD45RA的阳性率较低。在效靶比为10:1/20:1/50:1时，DC-CIK组的杀伤率高于DC组和CIK组（P<0.05）。治疗后，化疗组、DC组和CIK组大鼠体重显著降低（P<0.05），而阴性对照组和DC-CIK组未见明显变化（P>0.05）。治疗25天后，DC-CIK组肿瘤大小明显小于阴性对照组、化疗组、DC组和CIK组；DC-CIK组肿瘤组织坏死面积也明显大于阴性对照组、化疗组、DC组和CIK组（P<0.05）。DC与CIK共培养在增强CIK细胞增殖、增加IL-12和IFN-γ分泌、增强免疫细胞活性及抗肿瘤能力方面表现优异，显示出其在抗PCa肿瘤免疫治疗中的潜力。
