Li Chunrong, Cao Fangzheng, Zhang Houwen, Fan Weijiao, Cheng Yifan, Lou Yao, Wang Yiqi
Center for rehabilitation Medicine (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China.
The Second Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, China.
Transl Neurosci. 2024 Oct 24;15(1):20220355. doi: 10.1515/tnsci-2022-0355. eCollection 2024 Jan 1.
Neuropathic pain is a common symptom of Guillain-Barré syndrome (GBS). The infiltration of macrophages in the dorsal root ganglion (DRG) contributed to neuropathic pain in nerve injury. The underlying mechanisms of neuropathic pain in patients with GBS remain unknown. Experimental autoimmune neuritis (EAN) is a useful mice model of GBS. Our study aimed to explore whether the infiltration of macrophages in DRG is associated with neuropathic pain of EAN.
Male C57BL/6 mice were randomly divided into two groups, the EAN group ( = 12) and the control group ( = 12). Six mice in each group were sacrificed after anesthetization in the attack and remission phase, respectively. The 50% paw withdrawal threshold and clinical score were measured, and macrophages with its subtypes were detected in the spleen and DRG tissue.
More macrophages infiltrated the DRG of the EAN group in the attack phase and mostly surrounded neurons in the DRG. The proportion of macrophages and pro-inflammatory macrophages in the spleen of mice with EAN was significantly higher than the control group in the attack phase.
The infiltration of macrophages in DRG might be associated with neuropathic pain of EAN and pro-inflammatory macrophages may involve in neuropathic pain of EAN.
神经性疼痛是吉兰-巴雷综合征（GBS）的常见症状。背根神经节（DRG）中巨噬细胞的浸润导致神经损伤中的神经性疼痛。GBS患者神经性疼痛的潜在机制尚不清楚。实验性自身免疫性神经炎（EAN）是GBS的一种有用的小鼠模型。我们的研究旨在探讨DRG中巨噬细胞的浸润是否与EAN的神经性疼痛相关。
将雄性C57BL/6小鼠随机分为两组，EAN组（n = 12）和对照组（n = 12）。每组各6只小鼠分别在发作期和缓解期麻醉后处死。测量50%缩爪阈值和临床评分，并在脾脏和DRG组织中检测巨噬细胞及其亚型。
发作期EAN组DRG中有更多巨噬细胞浸润，且大多围绕DRG中的神经元。EAN小鼠脾脏中巨噬细胞和促炎巨噬细胞的比例在发作期显著高于对照组。
DRG中巨噬细胞的浸润可能与EAN的神经性疼痛相关，促炎巨噬细胞可能参与EAN的神经性疼痛。
