Dyrholm Rasmus Majland, Simonsen Pernille Arent, Pertoldi Cino, Schou Toke Munk, Muurmann Asmus Toftkær, Bahrndorff Simon
Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark.
Aalborg Zoo, Mølleparkvej 63, 9000 Aalborg, Denmark.
Insects. 2024 Oct 1;15(10):764. doi: 10.3390/insects15100764.
Increasing human population size and income growth are causing an increasing demand for food and feed. Insects are a more sustainable alternative to conventional animal source proteins, as they can convert waste and by-products from the agricultural industry into biomass for commercial feed for livestock and, potentially, serve as a food source for human consumption. Moreover, insects together with their microorganisms have been shown to play a pivotal role in the development of insects and in the breakdown of complex growth substrates, and are, therefore, closely tied to insect production. This study aims to determine if the removal of egg- and substrate-associated microorganisms impacts larval performance (growth, final biomass, and the survival rate) of Linnaeus. Four treatments are tested: disinfected eggs and non-autoclaved substrate, non-disinfected eggs and autoclaved substrate, disinfected eggs and autoclaved substrate, and a control without any removal of microbiota. The results show a significant decrease in the final biomass of larvae subjected to the treatments with only disinfected eggs, only autoclaved substrate, and both compared to the control, and a significant decrease in survival rate for non-disinfected eggs and autoclaved substrate and disinfected eggs and autoclaved substrate compared to the control group. Moreover, larval growth shows a significant difference across days within all treatments. Together, this suggests that the microorganisms of housefly eggs and the growth substrate play an important role in biomass, which is critical in commercial insect production. Together this suggest, that more studies are needed to examine these parameters with respect to more commercially relevant substrates.
人类人口规模的不断增长和收入的增加导致对食物和饲料的需求日益增长。昆虫是传统动物源蛋白质更具可持续性的替代品，因为它们可以将农业产业的废弃物和副产品转化为用于牲畜商业饲料的生物质，并且有可能成为人类消费的食物来源。此外，昆虫及其微生物已被证明在昆虫发育和复杂生长底物的分解中起着关键作用，因此与昆虫生产密切相关。本研究旨在确定去除与卵和底物相关的微生物是否会影响家蝇的幼虫性能（生长、最终生物量和存活率）。测试了四种处理方法：消毒卵和未高压灭菌的底物、未消毒卵和高压灭菌的底物、消毒卵和高压灭菌的底物，以及不进行任何微生物去除的对照组。结果表明，与对照组相比，仅用消毒卵处理、仅用高压灭菌底物处理以及两者都用处理的幼虫最终生物量显著降低，并且与对照组相比，未消毒卵和高压灭菌底物以及消毒卵和高压灭菌底物处理的幼虫存活率显著降低。此外，在所有处理中，幼虫生长在不同天数之间存在显著差异。总之，这表明家蝇卵和生长底物的微生物在生物量方面起着重要作用，这在商业昆虫生产中至关重要。这共同表明，需要更多研究来针对更具商业相关性的底物研究这些参数。
