Feng Lichen, Hu Lianrong, Bo Jingyi, Ji Mei, Ze Sangzi, Ding Yan'e, Yang Bin, Zhao Ning
College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.
Yunnan Academy of Forestry and Grassland, Kunming 650224, China.
J Fungi (Basel). 2024 Oct 3;10(10):691. doi: 10.3390/jof10100691.
is one of the most threatening invasive plant species in the world. Its invasion has greatly reduced the species diversity of the invaded areas. The development of fungal herbicides using phytopathogenic fungi has attracted considerable attention in recent years. In this study, a tissue isolation method was used to isolate and screen the strain SWFU-MM002 with strong pathogenicity to leaves from naturally occurring . Through morphological observation, , , and gene sequence homology, we compare and construct a phylogenetic tree to determine their taxonomic status. In addition, the biological characteristics of strain SWFU-MM002 were studied. The results showed that, combined with morphological and molecular biology identification, the strain was identified as ; biological characteristic research showed that the optimal medium for the growth of mycelium of this strain is PDA medium. At the optimal temperature of 27 °C and pH between 6 and 10, the mycelium can grow well. The best carbon and nitrogen sources are maltose and peptone, respectively. Analysing the infection process under a light microscope showed that SWFU-MM002 mycelia invaded the leaf tissue through stomata and colonized, eventually causing damage to the host. This is the first report of leaf spot of caused by . This study can lay a solid foundation for the development of as a control agent for .
是世界上最具威胁的入侵植物物种之一。其入侵极大地降低了入侵地区的物种多样性。近年来,利用植物病原真菌开发真菌除草剂受到了广泛关注。在本研究中,采用组织分离法从自然发生的[植物名称]叶片中分离并筛选出对其具有强致病性的菌株SWFU-MM002。通过形态观察、[其他鉴定方法1]、[其他鉴定方法2]和[基因名称]基因序列同源性分析,我们进行比较并构建系统发育树以确定它们的分类地位。此外,还对菌株SWFU-MM002的生物学特性进行了研究。结果表明,结合形态学和分子生物学鉴定,该菌株被鉴定为[具体菌种名称];生物学特性研究表明,该菌株菌丝生长的最佳培养基是PDA培养基。在27℃的最佳温度和pH值在6至10之间时,菌丝可以良好生长。最佳碳源和氮源分别是麦芽糖和蛋白胨。在光学显微镜下分析感染过程表明,SWFU-MM002菌丝通过气孔侵入叶片组织并定殖,最终对寄主造成损害。这是关于由[具体菌种名称]引起的[植物名称]叶斑病的首次报道。本研究可为开发[具体菌种名称]作为[植物名称]的防治剂奠定坚实基础。