Ultra-High-Performance Liquid Chromatography-High-Definition Mass Spectrometry-Based Metabolomics to Reveal the Potential Anti-Arthritic Effects of in Cultured Fibroblast-like Synoviocytes Derived from Rheumatoid Arthritis.

BACKGROUND

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. The fruits of , which is a medicinal and edible resource, have been shown to have anti-inflammatory properties.

METHODS

In this study, we investigated the effects of extracts (IVEs) on human RA fibroblasts-like synoviocytes (RA-FLS) by using a sensitive and selective ultra-high-performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) method. We subsequently analyzed the metabolites produced after the incubation of cultured RA-FLS with IVEs.

RESULTS

IVEs inhibited the proliferation and suppressed the migration of RA-FLS, and reduced the levels of inflammatory factors including TNF-α and IL-6. Twenty differential metabolites responsible for the effects of IVEs were screened and annotated based on the UPLC-HDMS data by using a cell metabolomics approach.

DISCUSSION

Our findings suggest that treating RA-FLS with IVEs can regulate lipid and amino acid metabolism, indicating that this extract has the potential to modify the metabolic pathways that cause inflammation in RA.

CONCLUSIONS

This might lead to novel therapeutic strategies for managing patients with RA.