Food Research Center (FoRC), Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo (USP), São Paulo, SP, Brazil.
Microbiology Department, Institute of Biological Sciences, University of São Paulo (USP), São Paulo, SP, Brazil.
Microb Pathog. 2024 Dec;197:107079. doi: 10.1016/j.micpath.2024.107079. Epub 2024 Oct 23.
Bacteria coordinate gene expression in a cell density-dependent manner using a communication process called quorum sensing (QS). The expression of virulence factors, biofilm formation and enzyme production are examples of QS-regulated phenotypes that can interfere with food quality and safety. Due to the importance of these phenotypes, the inhibition of bacterial communication as an anti-virulence strategy is of great interest. This work aimed to evaluate the effect of phenolic compounds on the inhibition of biofilm formation by Pseudomonas aeruginosa PAO1, using concentrations that do not interfere in bacterial growth. The synergistic effect of rosmarinic acid, baicalein, curcumin and resveratrol with tobramycin and between the phenolics themselves was evaluated. The tested combinations proved to be a good strategy for reducing the dose of antibiotics used in treatments and obtaining satisfactory results against P. aeruginosa biofilms. The combination of the four compounds at the highest concentration (500 μM) completely inhibited biofilm formation. The obtained results contribute to understanding the effect of phenolic compounds on QS inhibition, which may help to define the mechanism of inhibition, in addition to expanding the biotechnological potential of these compounds for future applications in the food, pharmaceutical and medical fields.
细菌通过一种称为群体感应 (QS) 的通信过程，以细胞密度依赖的方式协调基因表达。毒力因子的表达、生物膜形成和酶的产生是 QS 调节表型的例子，它们会干扰食品质量和安全。由于这些表型的重要性，抑制细菌通信作为一种抗毒力策略具有重要意义。本工作旨在评估酚类化合物对铜绿假单胞菌 PAO1 生物膜形成的抑制作用，使用的浓度不会干扰细菌生长。评估了迷迭香酸、黄芩素、姜黄素和白藜芦醇与妥布霉素的协同作用以及酚类化合物之间的协同作用。测试的组合被证明是减少抗生素在治疗中使用剂量并获得对铜绿假单胞菌生物膜令人满意结果的良好策略。四种化合物在最高浓度（500 μM）下的组合完全抑制了生物膜的形成。所得结果有助于理解酚类化合物对 QS 抑制的影响，这除了可以扩展这些化合物在食品、制药和医疗领域未来应用的生物技术潜力外，还有助于确定抑制机制。
