Salica Andrea, Cammisotto Vittoria, Scaffa Raffaele, Folino Giulio, De Paulis Ruggero, Carnevale Roberto, Benedetto Umberto, Saade Wael, Marullo Antonino, Sciarretta Sebastiano, Sarto Gianmarco, Palmerio Silvia, Valenti Valentina, Peruzzi Mariangela, Miraldi Fabio, Irace Francesco Giosuè, Frati Giacomo
Department of Cardiac Surgery, European Hospital, 00149 Rome, Italy.
Department of Clinical, Internal Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, Viale del Policlinico, 155, 00161 Rome, Italy.
Antioxidants (Basel). 2024 Sep 28;13(10):1180. doi: 10.3390/antiox13101180.
Oxidative stress and inflammation are typically implied in atherosclerosis pathogenesis and progression, especially in coronary artery disease (CAD). Our objective was to investigate the oxidative stress and inflammation burden directly associated with atherosclerotic plaque in patients with stable coronary disease undergoing coronary artery bypass graft (CABG) surgery. Specifically, markers of oxidative stress and inflammation were compared in blood samples obtained from the atherosclerotic left anterior descending artery (LAD) and blood samples obtained from the healthy left internal thoracic artery (LITA), used as a bypass graft, within the same patient.
Twenty patients scheduled for off-pump CABG were enrolled. Blood samples were collected from the LITA below anastomosis and the LAD below the stenosis. Samples were analysed for oxidative stress (sNOXdp, HO, NO) and inflammation markers (TNFα, IL-6, IL-1β, IL-10).
The analysis showed a significant increase in oxidative stress burden in the LAD as compared to LITA, as indicated by higher sNOX2-dp and HO levels and lower NO levels ( < 0.01). Also, pro-inflammatory cytokines were increased in the LAD as compared to the LITA, as indicated by higher TNFα and IL-6 amounts ( < 0.01). On the other hand, no significant differences could be seen regarding IL-1β and IL-10 levels between the two groups.
The oxidative stress and inflammatory burden are specifically enhanced in the LAD artery of stable coronary patients compared to systemic blood from the LITA of stable coronary patients.
氧化应激和炎症通常与动脉粥样硬化的发病机制及进展相关，尤其是在冠状动脉疾病（CAD）中。我们的目的是研究接受冠状动脉搭桥术（CABG）的稳定型冠心病患者中，与动脉粥样硬化斑块直接相关的氧化应激和炎症负担。具体而言，在同一患者体内，比较从动脉粥样硬化的左前降支（LAD）采集的血样和作为旁路移植血管的健康左内乳动脉（LITA）采集的血样中氧化应激和炎症标志物。
招募20例计划进行非体外循环CABG的患者。在吻合口下方采集LITA的血样，在狭窄下方采集LAD的血样。对样本进行氧化应激（sNOXdp、HO、NO）和炎症标志物（TNFα、IL-6、IL-1β、IL-10）分析。
分析显示，与LITA相比，LAD中的氧化应激负担显著增加，表现为sNOX2-dp和HO水平较高，而NO水平较低（<0.01）。此外，与LITA相比，LAD中的促炎细胞因子增加，表现为TNFα和IL-6含量较高（<0.01）。另一方面，两组之间IL-1β和IL-10水平未见显著差异。
与稳定型冠心病患者LITA的全身血液相比，稳定型冠心病患者LAD动脉中的氧化应激和炎症负担明显增强。
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