Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Pharmacognosy Department, Faculty of Pharmacy, Horus University in Egypt (HUE), New Damietta 34517, Egypt.
Int J Mol Sci. 2024 Oct 15;25(20):11077. doi: 10.3390/ijms252011077.
This study evaluated the antioxidant and antibacterial properties of methanolic extracts derived from oilseed cakes of (lettuce), (black seed), (rocket), and (linseed). Lettuce methanolic extract showed the highest potential, so it was selected for further investigation. High-performance liquid chromatography (HPLC-DAD) analysis and bioassay-guided fractionation of lettuce seed cake extract led to the isolation of five compounds: 1,3-propanediol-2-amino-1-(3',4'-methylenedioxyphenyl) (), luteolin (), luteolin-7-O--D-glucoside (), apigenin-7-O--D-glucoside (), and -sitosterol 3-O--D-glucoside (). Compound () was identified from species for the first time, with high yield. The cytotoxic effects of the isolated compounds were tested on liver (HepG2) and breast (MCF-7) cancer cell lines, compared to normal cells (WI-38). Compounds (), (), and () exhibited strong activity in all assays, while compound () showed weak antioxidant, antimicrobial, and cytotoxic effects. The anti-inflammatory activity of lettuce seed cake extract and compound () was evaluated in vivo using a carrageenan-induced paw oedema model. Compound () and its combination with ibuprofen significantly reduced paw oedema, lowered inflammatory mediators (IL-1β, TNF-α, PGE2), and restored antioxidant enzyme activity. Additionally, compound () showed promising COX-1 and COX-2 inhibition in an in vitro enzymatic anti-inflammatory assay, with IC values of 17.31 ± 0.65 and 4.814 ± 0.24, respectively. Molecular docking revealed unique interactions of compound () with COX-1 and COX-2, suggesting the potential for targeted inhibition. These findings underscore the value of oilseed cakes as a source of bioactive compounds that merit further investigation.
本研究评估了来自 （生菜）、 （黑种草）、 （火箭菜）和 （亚麻籽）油籽饼的甲醇提取物的抗氧化和抗菌性能。生菜甲醇提取物表现出最高的潜力，因此被选来进行进一步的研究。高效液相色谱（HPLC-DAD）分析和生物活性导向的生菜籽饼提取物分离导致分离出五种化合物：1,3-丙二醇-2-氨基-1-（3',4'-亚甲二氧基苯基）（）、叶黄素（）、叶黄素-7-O--D-葡萄糖苷（）、芹菜素-7-O--D-葡萄糖苷（）和 -谷甾醇 3-O--D-葡萄糖苷（）。化合物（）是首次从 种中鉴定出来的，产量很高。将分离得到的化合物的细胞毒性作用测试在肝癌（HepG2）和乳腺癌（MCF-7）癌细胞系以及正常细胞（WI-38）上进行。化合物（）、（）和（）在所有试验中均表现出强烈的活性，而化合物（）则表现出弱的抗氧化、抗菌和细胞毒性作用。生菜籽饼提取物和化合物（）的抗炎活性通过角叉菜胶诱导的爪肿胀模型在体内进行评估。化合物（）及其与布洛芬的组合显著减轻了爪肿胀，降低了炎症介质（IL-1β、TNF-α、PGE2），并恢复了抗氧化酶活性。此外，化合物（）在体外酶抗炎测定中表现出对 COX-1 和 COX-2 的有希望的抑制作用，IC 值分别为 17.31±0.65 和 4.814±0.24。分子对接显示化合物（）与 COX-1 和 COX-2 具有独特的相互作用，表明其具有靶向抑制的潜力。这些发现强调了油籽饼作为具有生物活性化合物的来源的价值，值得进一步研究。
