Zhang Danmei, Guo Jin, Shi Chunxia, Wang Yukun, Zhang Yanqiong, Zhang Xiaoya, Gong Zuojiong
Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Biomedicines. 2024 Sep 26;12(10):2190. doi: 10.3390/biomedicines12102190.
Neutrophils, as the first line of defense in the immune response, produce neutrophil extracellular traps (NETs) upon activation, which are significant in the pathogenesis and organ damage in sepsis. This study aims to explore the clinical value of myeloperoxidase-DNA (MPO-DNA) and cell-free DNA (cf-DNA) in sepsis patients. Clinical data were collected from 106 sepsis patients, 25 non-sepsis patients, and 51 healthy controls. Sequential Organ Failure Assessment (SOFA) scores were calculated, and levels of MPO-DNA) complexes and cf-DNA were measured using specific kits. Correlation analyses assessed relationships between indicators, while logistic regression identified independent risk factors. Receiver operating characteristic (ROC) curves calculated the area under the curve (AUC) to evaluate the diagnostic value of the biomarkers. Sepsis patients exhibited significantly elevated levels of MPO-DNA and cf-DNA compared to non-sepsis patients and healthy controls. In sepsis patients, MPO-DNA and cf-DNA levels correlated with inflammation, coagulation, and organ damage indicators, as well as procalcitonin (PCT) levels and SOFA scores. Both C-reactive protein (CRP) and cf-DNA were identified as independent risk factors for sepsis, demonstrating moderate diagnostic value. ROC analysis showed that the combination of MPO-DNA and CRP (AUC: 0.837) enhances the AUC value of CRP (0.777). In summary, elevated serum levels of MPO-DNA and cf-DNA in sepsis patients correlate with SOFA scores and PCT levels, providing reference value for sepsis diagnosis in clinical settings.
中性粒细胞作为免疫反应的第一道防线，激活后会产生中性粒细胞胞外诱捕网（NETs），这在脓毒症的发病机制和器官损伤中具有重要意义。本研究旨在探讨髓过氧化物酶 - DNA（MPO - DNA）和游离DNA（cf - DNA）在脓毒症患者中的临床价值。收集了106例脓毒症患者、25例非脓毒症患者和51例健康对照者的临床资料。计算序贯器官衰竭评估（SOFA）评分，并使用特定试剂盒测量MPO - DNA复合物和cf - DNA的水平。相关性分析评估指标之间的关系，而逻辑回归确定独立危险因素。受试者工作特征（ROC）曲线计算曲线下面积（AUC）以评估生物标志物的诊断价值。与非脓毒症患者和健康对照相比，脓毒症患者的MPO - DNA和cf - DNA水平显著升高。在脓毒症患者中，MPO - DNA和cf - DNA水平与炎症、凝血和器官损伤指标以及降钙素原（PCT）水平和SOFA评分相关。C反应蛋白（CRP）和cf - DNA均被确定为脓毒症的独立危险因素，具有中等诊断价值。ROC分析表明，MPO - DNA和CRP联合使用（AUC：0.837）提高了CRP的AUC值（0.777）。总之，脓毒症患者血清中MPO - DNA和cf - DNA水平升高与SOFA评分和PCT水平相关，为临床脓毒症诊断提供参考价值。
