Guamán Santiago A, Elhadi Abdelaali, Salama Ahmed A K, Manuelian Carmen L, Caja Gerardo, Albanell Elena
Ruminant Research Group (G2R), Department of Animal and Food Sciences, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
Sede Orellana, Escuela Superior Politécnica de Chimborazo (ESPOCH), El Coca 220150, Ecuador.
Animals (Basel). 2024 Oct 18;14(20):3023. doi: 10.3390/ani14203023.
This study evaluated short-term immune responses of dairy ewes supplemented with barley β-glucan (BG) following an intramammary lipopolysaccharide (LPS) challenge. In the adaptation period, 36 ewes were fed an alfalfa hay diet ad libitum and barley grain cv. Hispanic (3.8% BG). Then, ewes were assigned into three experimental groups: (1) Control (CON), the same previous diet (13.3 g BG/d); (2) high β-glucans barley (HBG), new barley (cv. Annapurna) containing 10% BG (35 g BG/d); (3) intraperitoneally injected (INP) with a 1.4% BG solution dose (2 g BG/ewe). At d 9, all ewes were infused with an LPS or saline solution in each udder half. After the challenge, rectal temperature (RT), milk yield and composition, somatic cell count (SCC), and plasma interleukins (IL-1α and IL-1β) were monitored daily. The INP treatment revealed a transitory increase in RT and decreased milk yield by 38%. Milk fat, protein, and SCC increased in LPS-treated udders but not by BG treatment. The IL-1α plasma concentration was similar among groups but INP ewes showed a lower IL-1β concentration suggesting a lower inflammatory response. The BG administration appears more effective intraperitoneally than orally, which needs additional study.
本研究评估了在乳腺内注射脂多糖（LPS）攻击后，补充大麦β-葡聚糖（BG）的奶羊的短期免疫反应。在适应期，36只母羊自由采食苜蓿干草日粮和大麦品种Hispanic（BG含量3.8%）。然后，将母羊分为三个实验组：（1）对照组（CON），饲喂与之前相同的日粮（13.3克BG/天）；（2）高β-葡聚糖大麦组（HBG），饲喂含10%BG的新大麦（品种Annapurna，35克BG/天）；（3）腹腔注射（INP）1.4%BG溶液剂量（2克BG/只母羊）。在第9天，给所有母羊每个乳房的一半注入LPS或生理盐水溶液。攻击后，每天监测直肠温度（RT）、产奶量和成分、体细胞计数（SCC）以及血浆白细胞介素（IL-1α和IL-1β）。INP处理显示RT出现短暂升高，产奶量下降38%。LPS处理的乳房中乳脂肪、蛋白质和SCC增加，但BG处理未使其增加。各组间IL-1α血浆浓度相似，但INP组母羊的IL-1β浓度较低，表明炎症反应较低。BG腹腔注射给药似乎比口服给药更有效，这需要进一步研究。
