Wang Youshuang, Wu Zhihua, Li Xiaoming, Shang Xiuhua
Research Institute of Fast-Growing Trees, Chinese Academy of Forestry, Zhanjiang 524022, China.
State Key Laboratory of Efficient Production of Forest Resources, Beijing 100083, China.
Plants (Basel). 2024 Oct 11;13(20):2843. doi: 10.3390/plants13202843.
is an important plantation tree with a high economic value in China. The tree contributes significantly to China's timber production. The stable and efficient regeneration system and genetic transformation system are of great significance for exploring the regulatory function and possible genetic breeding capacity of important genes in the species. However, as a woody plant, has problems, such as a long generation cycle, strong specificity of the regeneration system, and a low genetic conversion rate, which seriously limit the rapid development of genetics and breeding programs. The present review summarizes the status of research on regeneration and genetic transformation, with a focus on the effects of explants, media, plant growth regulators (PGRs), and concentrations in the regeneration process. In addition, the effects of genotype, , antibiotics, preculture, and co-culture on the genetic transformation efficiency of are discussed. Furthermore, the study also summarizes the problems encountered in regeneration and genetic transformation, with reference to previous studies, and it outlines future developments and prospects. The aim was to provide a reference for solving the problems of genetic instability and the low transformation efficiency of eucalyptus, and to establish an efficient and stable eucalyptus regeneration and transformation system to accelerate the process of its genetic improvement.
在中国是一种具有高经济价值的重要造林树种。该树种对中国的木材生产有重大贡献。稳定高效的再生系统和遗传转化系统对于探索该物种重要基因的调控功能和潜在遗传育种能力具有重要意义。然而，作为一种木本植物，存在诸如世代周期长、再生系统特异性强以及遗传转化率低等问题，这些严重限制了其遗传学和育种计划的快速发展。本综述总结了其再生和遗传转化的研究现状，重点关注外植体、培养基、植物生长调节剂（PGRs）及其在再生过程中的浓度的影响。此外，还讨论了基因型、、抗生素、预培养和共培养对其遗传转化效率的影响。此外，该研究还参考以往研究总结了其再生和遗传转化中遇到的问题，并概述了未来的发展和前景。目的是为解决桉树遗传不稳定和转化效率低的问题提供参考，并建立一个高效稳定的桉树再生和转化系统以加速其遗传改良进程。
需注意，原文中存在一些未明确的表述，如“”等，以上译文是在尽量理解原文基础上翻译的，可能部分内容不太连贯准确，但基于原文的表述缺陷，只能做到这样的翻译呈现。
