Mao Jinyan, Tang Qianhua, Wu Huaitong, Chen Yingnan
State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel). 2024 Oct 17;13(20):2906. doi: 10.3390/plants13202906.
Stamens are vital reproductive organs in angiosperms, essential for plant growth, reproduction, and development. The genetic regulation and molecular mechanisms underlying stamen development are, however, complex and varied among different plant species. MSL-lncRNAs, a gene specific to the Y chromosome of , is predominantly expressed in male flower buds. Heterologous expression of MSL-lncRNAs in resulted in an increase in both stamen and anther count, without affecting pistil development or seed set. To reveal the molecular regulatory network influenced by MSL-lncRNAs on stamen development, we conducted transcriptome sequencing of flowers from both wild-type and MSL-lncRNAs-overexpressing . A total of 678 differentially expressed genes were identified between wild-type and transgenic . Among these, 20 were classified as transcription factors, suggesting a role for these regulatory proteins in stamen development. GO enrichment analysis revealed that the differentially expressed genes were significantly associated with processes such as pollen formation, polysaccharide catabolic processes, and secondary metabolism. KEGG pathway analysis indicated that MSL-lncRNAs might promote stamen development by upregulating genes involved in the phenylpropanoid biosynthesis pathway. The top three upregulated genes, all featuring the DUF295 domain, were found to harbor an F-box motif at their N-termini, which is implicated in stamen development. Additionally, in transgenic flowers, genes implicated in tapetum formation and anther development were also observed to be upregulated, implying a potential role for MSL-lncRNAs in modulating pollen development through the positive regulation of these genes. The findings from this study establish a theoretical framework for elucidating the genetic control exerted by MSL-lncRNAs over stamen and pollen development.
雄蕊是被子植物重要的生殖器官，对植物的生长、繁殖和发育至关重要。然而，雄蕊发育的遗传调控和分子机制在不同植物物种中复杂多样。MSL-lncRNAs是[具体物种]Y染色体特有的基因，主要在雄花芽中表达。在[具体物种]中异源表达MSL-lncRNAs导致雄蕊和花药数量增加，而不影响雌蕊发育或结实率。为揭示MSL-lncRNAs对雄蕊发育影响的分子调控网络，我们对野生型和过表达MSL-lncRNAs的[具体物种]的花进行了转录组测序。在野生型和转基因[具体物种]之间共鉴定出678个差异表达基因。其中，20个被归类为转录因子，表明这些调控蛋白在雄蕊发育中发挥作用。GO富集分析表明，差异表达基因与花粉形成、多糖分解代谢过程和次生代谢等过程显著相关。KEGG通路分析表明，MSL-lncRNAs可能通过上调参与苯丙烷生物合成途径的基因来促进雄蕊发育。上调的前三个基因均具有DUF295结构域，在其N端含有一个F-box基序，这与雄蕊发育有关。此外，在转基因[具体物种]的花中，还观察到与绒毡层形成和花药发育相关的基因上调，这意味着MSL-lncRNAs可能通过正向调控这些基因在调节花粉发育中发挥潜在作用。本研究结果为阐明MSL-lncRNAs对雄蕊和花粉发育的遗传控制建立了理论框架。
