Nuclear Institute for Agriculture and Biology College, Pakistan Institute of Engineering and Applied Sciences (NIAB-C, PIEAS), Faisalabad, Pakistan.
Sci Rep. 2024 Oct 29;14(1):25942. doi: 10.1038/s41598-024-77204-0.
Cotton (Gossypium hirsutum L.) is a multipurpose crop. Abiotic stresses, especially extreme heat and drought, limit crop growth and thus reduce cotton yield by about 50%. In this study, 30 cotton genotypes were tested against low moisture stress in a pot experiment in triplicates along with control under wire house conditions. At the 3-4 leaf stage, different morpho-physiological and biochemical parameters were measured in order to select the low moisture stress-tolerant genotypes. For the selection of the best performing genotypes, Multi-Trait Genotype-Ideotype Distance Index (MGIDI) was used for the ranking of genotypes on the basis of multiple indices. For biochemical traits, 09 (TPC, TF, TSP, MDA, SOD, POD, CAT, APX, and Proline) out of 24 showed significant genotypic effects and were used for MGIDI. Eight genotypes (N-812 N-1296 N-696 N-377 N-121-896 N-T86, and N-3496) were observed to be best performing than others at 25% selection pressure (SI = 25%). For morpho-physiological traits, 14 out of 15 showed significant genotypic effects and used for MGIDI. Ten genotypes (N-1237 N-812 N-1296 N-696 N-9078 N-377 N-512 N-121 N-375, and N-896) were observed to be best performing at 35% selection pressure (SI = 35%). Six genotypes, i.e. N-812-1296 N-696 N-377 N-121, and N-896 were found common in both MGIDI analysis. In conclusion, three genotypes, i.e. N-696, N-896, and N-T86 proved to be most resilient to low moisture stress. Develop protocols, identified genotypes and markers that can be used for development of climate-smart cotton genotypes.
棉花（Gossypium hirsutum L.）是一种多用途作物。非生物胁迫，特别是极端高温和干旱，限制了作物的生长，从而使棉花产量减少约 50%。在这项研究中，30 个棉花基因型在温室条件下的盆栽试验中进行了 3 次重复，同时进行了对照试验。在 3-4 叶期，测量了不同的形态生理和生化参数，以选择对低水分胁迫具有耐受性的基因型。为了选择表现最好的基因型，使用多性状基因型-理想型距离指数（MGIDI）根据多个指标对基因型进行排名。对于生化性状，24 个中有 09 个（TPC、TF、TSP、MDA、SOD、POD、CAT、APX 和脯氨酸）表现出显著的基因型效应，并用于 MGIDI。在 25%的选择压力（SI=25%）下，观察到 8 个基因型（N-812、N-1296、N-696、N-377、N-121-896、N-T86 和 N-3496）比其他基因型表现更好。对于形态生理性状，15 个中有 14 个表现出显著的基因型效应，并用于 MGIDI。在 35%的选择压力（SI=35%）下，观察到 10 个基因型（N-1237、N-812、N-1296、N-696、N-9078、N-377、N-512、N-121、N-375 和 N-896）表现更好。在两种分析中，发现了 6 个共同的基因型，即 N-812-1296、N-696、N-377、N-121 和 N-896。综上所述，N-696、N-896 和 N-T86 这 3 个基因型对低水分胁迫最具弹性。制定协议，确定基因型和标记，可用于开发抗逆棉花基因型。
