Characterization of Endophytic Ahn75 and Its Potential for Biocontrol against Rice Blast.

Plant endophyte Streptomyces are excellent candidates as biocontrol agents against the rice blast fungus, . In this study, a novel strain Ahn75 with antifungal activity was isolated from healthy rice stem and identified as by phenotypic characterization and phylogenetic analysis based on 16S rRNA gene, multilocus and genome sequences. Inhibition test using culture filtrate showed that Ahn75 could effectively suppress , with mycelia growth inhibition rate of 80.88% and spore germination inhibition rate of 78.26%. Genome sequence analysis of strain Ahn75 showed 40 gene clusters of secondary metabolites and several genes related to plant growth promotion were predicted in the genome of Ahn75. Several antimicrobial compounds including valinomycin, tetrabutylammonium, and benzalkonium chloride, were also detected in the antifungal fraction from Ahn75 culture filtrate by liquid chromatography and high resolution mass spectrometry. Meanwhile, strain Ahn75 demonstrates UV tolerance under UV irradiation for 60 min, pH tolerance between pH6 and pH9, and a high halotolerance in 7% (w/v) of NaCl. Greenhouse experiments indicated that Ahn75 is able to colonize rice stems, roots, and leaves, which help rice to reduce the rice leaf blast incidence by 59.76%. All these findings suggest that strain Ahn75 could be a potential biocontrol agent for rice blast.