Halsell S R, Jeffery W R
Biochem Biophys Res Commun. 1986 Jan 29;134(2):469-76. doi: 10.1016/s0006-291x(86)80444-2.
Translational regulation of protein synthesis accompanies sclerotization in Physarum polycephalum. Plasmodial and sclerotial poly(A)+ RNA were translated in a message-dependent wheat germ lysate in the presence of the cap analogue 7-methylguanosine-triphosphate to determine whether 5' structural alterations in mRNA accompany translational repression. The translation of plasmodial and sclerotial poly(A)+ RNA was reduced to identical levels suggesting that both RNA populations are capped. The 5'-termini of plasmodial and sclerotial poly(A)+ RNA were identified as m7G5'ppp5'Cm. Alterations in the 5'-cap of mRNA during sclerotization do not appear to be responsible for translational dormancy.
多头绒泡菌的蛋白质合成的翻译调控伴随着硬化过程。在帽类似物7-甲基鸟苷三磷酸存在的情况下，在依赖信使的小麦胚芽裂解物中翻译原质团和硬化体的聚腺苷酸（poly(A)）+ RNA，以确定mRNA中5'结构改变是否伴随着翻译抑制。原质团和硬化体的聚腺苷酸（poly(A)）+ RNA的翻译都降低到相同水平，这表明这两种RNA群体都有帽结构。原质团和硬化体的聚腺苷酸（poly(A)）+ RNA的5'末端被鉴定为m7G5'ppp5'Cm。硬化过程中mRNA的5'-帽结构改变似乎不是翻译休眠的原因。
